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CaMKII alpha (phospho Thr286) antibody [22B1]

Orthogonal
Anti-CaMKII alpha (phospho Thr286) antibody [22B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22724
Anti-CaMKII alpha (phospho Thr286) antibody [22B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22724
Anti-CaMKII alpha (phospho Thr286) antibody [22B1] used in Western Blot (WB). GTX22724

Cat. No. GTX22724

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 22B1

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Rabbit
Package
100 μl ($399)
See all CaMKII alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000
ICC/IF 1:50-1:100
FACS 1μg per 10⁶ cells
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Product Note

This antibody has been shown to detect the phosphorylated alpha subunit CaM kinase II only. It does not detect the non-phosphorylated alpha subunit CaM kinase II nor with the ~60 kDa beta subunit in either phosphorylation state. Immunofluorescence staining of CaM kinase II in rat/mouse hippocampal cells with GTX22724 densely labels cell somas and neuropil areas, while nuclei are only lightly stained.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues M(281) H R Q E T(p) V D C L K K F N(294) of rat CaM Kinase II protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin-dependent protein kinase II alpha , PK2CDD , PKCCD

Cellular Localization

Cell junction, synapse,Cell projection, dendrite

Database

Research Area

DATA IMAGES

Anti-CaMKII alpha (phospho Thr286) antibody [22B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22724

GTX22724 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-CaMKII alpha (phospho Thr286) antibody [22B1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22724

GTX22724 ICC/IF Image

Orthogonal

ICC/IF analysis of Neuro-2a cells (left panel) or cells stimulated with Lamda-Phosphatase (Lambda-PPase) (right panel) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute

Anti-CaMKII alpha (phospho Thr286) antibody [22B1] used in Western Blot (WB). GTX22724

GTX22724 WB Image

WB analysis of unphosphorylated and phosphorylated rat brain homogenate (left and right lanes respectively) using GTX22724 CaMKII alpha (phospho Thr286) antibody [22B1] or GTX22725CaMKII alpha antibody [6G9] .

REFERENCE

Application Reference

Lee LC et al. Mol Brain 2021; 14 (1) : 152 Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399