CaMKII alpha (phospho Thr286) antibody [HL3570]

CaMKII alpha (phospho Thr286) antibody [HL2908] detects CaMKII alpha (phospho Thr286) protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CaMKII alpha (phospho Thr286) stained by CaMKII alpha (phospho Thr286) antibody [HL2908] (GTX641517) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Specificity test was performed by coating the plate with CAMK2A peptide, CAMK2A (phospho Thr286) peptide, CAMK2B peptide, CAMK2B (phospho Thr287) peptide, CAMK2G peptide, and CAMK2G (phospho Thr287) peptide (125 ng/mL). Coated peptides were probed with CaMKII alpha (phospho Thr286) antibody [HL3570] (GTX641517) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) mouse brain extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII alpha (phospho Thr286) antibody [HL3570] (GTX641517) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.