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CaMKII alpha (phospho Thr286) antibody

Anti-CaMKII alpha (phospho Thr286) antibody used in Western Blot (WB). GTX133859

Cat. No. GTX133859

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all CaMKII alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Human, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr286 of human CaMKII alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase II alpha , CAMKA , CaMKIINalpha , MRD53 , MRT63

Cellular Localization

Cell junction , synapse , presynaptic cell membrane , synapse

Background

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CaMKII alpha (phospho Thr286) antibody used in Western Blot (WB). GTX133859

GTX133859 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII alpha (phospho Thr286) antibody (GTX133859) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang Z et al. EBioMedicine 2018; 36 : 54-62 TRPA1 inhibition ameliorates pressure overload-induced cardiac hypertrophy and fibrosis in mice.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169