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CaMKII antibody [GT4811]

Overexpression
Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127
Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127
Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127
Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127

Cat. No. GTX632127

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT4811

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CaMKII products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , Neuro 2A , C8D30 , NIH-3T3 , mouse brain , rat btain , transfected 293T

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKII. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888289

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127

GTX632127 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody (GTX632127) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127

GTX632127 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody (GTX632127) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127

GTX632127 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody (GTX632127) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CaMKII antibody [GT4811] used in Western Blot (WB). GTX632127

GTX632127 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [GT4811] (GTX632127) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lee LC et al. Mol Brain 2021; 14 (1) : 152 Association of CaMK2A and MeCP2 signaling pathways with cognitive ability in adolescents.
Application : WB
Reactivity : Human
Liu J et al. Cell Mol Gastroenterol Hepatol 2021; (Epub) ANKRD22 Drives Rapid Proliferation of Lgr5+ Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury.
Application : WB
Reactivity : Human
Buonarati O.R. et al. bioRxiv 2021; (Epub) Conserved and divergent features of neuronal CaMKII holoenzyme structure, function, and high-order assembly.
Application : WB
Chung CC et al. Lab Invest 2019; (Epub) Vascular endothelial growth factor enhances profibrotic activities through modulation of calcium homeostasis in human atrial fibroblasts.
Application : WB
Reactivity : Human
Imoto K et al. Pflugers Arch 2018; (Epub) Characterization of fibroblasts from hypertrophied right ventricle of pulmonary hypertensive rats.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations