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CaMKII antibody [HL1831]

Anti-CaMKII antibody [HL1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637556
Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556
Anti-CaMKII antibody [HL1831] used in IHC (Paraffin sections) (IHC-P). GTX637556
Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556
Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556
Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556

Cat. No. GTX637556

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1831

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:100000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CaMKII.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase II alpha , calcium/calmodulin dependent protein kinase II beta , calcium/calmodulin dependent protein kinase II delta , calcium/calmodulin dependent protein kinase II gamma , CAMKA , CaMKIINalpha , MRD53 , MRT63 , CAM2 , CAMK2 , CAMKB , MRD54 , CAMKD , CAMK , CAMK-II , CAMKG

Cellular Localization

Cell junction, synapse,Cell projection, dendrite,Cytoplasm, cytoskeleton

Database

Research Area

DATA IMAGES

Anti-CaMKII antibody [HL1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637556

GTX637556 ICC/IF Image

CaMKII antibody [HL1831] detects CaMKII protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CaMKII stained by CaMKII antibody [HL1831] (GTX637556) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556

GTX637556 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII antibody [HL1831] used in IHC (Paraffin sections) (IHC-P). GTX637556

GTX637556 IHC-P Image

CaMKII antibody [HL1831] detects CaMKII protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CaMKII stained by CaMKII antibody [HL1831] (GTX637556) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556

GTX637556 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556

GTX637556 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556

GTX637556 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations