APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:100000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human CaMKII.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium/calmodulin dependent protein kinase II alpha , calcium/calmodulin dependent protein kinase II beta , calcium/calmodulin dependent protein kinase II delta , calcium/calmodulin dependent protein kinase II gamma , CAMKA , CaMKIINalpha , MRD53 , MRT63 , CAM2 , CAMK2 , CAMKB , MRD54 , CAMKD , CAMK , CAMK-II , CAMKG
Cellular Localization
Cell junction, synapse,Cell projection, dendrite,Cytoplasm, cytoskeleton
Database
Research Area
DATA IMAGES
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/templates/default/js/lightbox/images/loading.gif "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
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GTX637556 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637556 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637556 ICC/IF Image
CaMKII antibody [HL1831] detects CaMKII protein by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CaMKII stained by CaMKII antibody [HL1831] (GTX637556) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637556 IHC-P Image
CaMKII antibody [HL1831] detects CaMKII protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.CaMKII stained by CaMKII antibody [HL1831] (GTX637556) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637556 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637556 WB Image
Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII antibody [HL1831] (GTX637556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for CaMKII antibody [HL1831] (GTX637556). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CaMKII antibody [HL1831] (GTX637556). Be the first to share your experience with this product.
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/small/GTX637556/GTX637556_T-44823_20221007_WB_M_R_22101718_942.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/small/GTX637556/GTX637556_T-44823_20221014_WB_B_22101718_330.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637556](/upload/website/prouct_img/small/GTX637556/GTX637556_44872_20221230_ICC_IF_22122901_134.webp "Anti-CaMKII antibody [HL1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637556")
![Anti-CaMKII antibody [HL1831] used in IHC (Paraffin sections) (IHC-P). GTX637556](/upload/website/prouct_img/small/GTX637556/GTX637556_T-44823_20221028_IHC-P_M_22112723_818.webp "Anti-CaMKII antibody [HL1831] used in IHC (Paraffin sections) (IHC-P). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/small/GTX637556/GTX637556_44872_20221125_WB_TPM_watermark_22112723_310.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/small/GTX637556/GTX637556_44872_20230324_WB_Drosophila_brain_23032819_746.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/normal/GTX637556/GTX637556_T-44823_20221007_WB_M_R_22101718_942.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/normal/GTX637556/GTX637556_T-44823_20221014_WB_B_22101718_330.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637556](/upload/website/prouct_img/normal/GTX637556/GTX637556_44872_20221230_ICC_IF_22122901_134.webp "Anti-CaMKII antibody [HL1831] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637556")
![Anti-CaMKII antibody [HL1831] used in IHC (Paraffin sections) (IHC-P). GTX637556](/upload/website/prouct_img/normal/GTX637556/GTX637556_T-44823_20221028_IHC-P_M_22112723_818.webp "Anti-CaMKII antibody [HL1831] used in IHC (Paraffin sections) (IHC-P). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/normal/GTX637556/GTX637556_44872_20221125_WB_TPM_watermark_22112723_310.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")
![Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556](/upload/website/prouct_img/normal/GTX637556/GTX637556_44872_20230324_WB_Drosophila_brain_23032819_746.webp "Anti-CaMKII antibody [HL1831] used in Western Blot (WB). GTX637556")