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CaMKII beta antibody

Anti-CaMKII beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX133072
Anti-CaMKII beta antibody used in IHC (Paraffin sections) (IHC-P). GTX133072
Anti-CaMKII beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133072
Anti-CaMKII beta antibody used in Western Blot (WB). GTX133072
Anti-CaMKII beta antibody used in Western Blot (WB). GTX133072

Cat. No. GTX133072

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CaMKII beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CaMKII beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886816

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase II beta , CAM2 , CAMK2 , CAMKB , MRD54

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CaMKII beta antibody used in IHC (Frozen sections) (IHC-Fr). GTX133072

GTX133072 IHC-Fr Image

CaMKII beta antibody detects CaMKII beta protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: CaMKII beta protein stained by CaMKII beta antibody (GTX133072) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CaMKII beta antibody used in IHC (Paraffin sections) (IHC-P). GTX133072

GTX133072 IHC-P Image

CaMKII beta antibody detects CAMK2B protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CaMKII beta antibody (GTX133072) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CaMKII beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133072

GTX133072 ICC/IF Image

CaMKII beta antibody detects CaMKII beta protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CaMKII beta protein stained by CaMKII beta antibody (GTX133072) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CaMKII beta antibody used in Western Blot (WB). GTX133072

GTX133072 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII beta antibody (GTX133072) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII beta antibody used in Western Blot (WB). GTX133072

GTX133072 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII beta antibody (GTX133072) diluted at 1:1000.

REFERENCE

Application Reference

Aoto K et al. Int J Mol Sci 2022; 23 (19) Generation of Flag/DYKDDDDK Epitope Tag Knock-In Mice Using i-GONAD Enables Detection of Endogenous CaMKIIα and β Proteins.
Islam R et al. Nutrients 2019; 11 (4) Theobromine Improves Working Memory by Activating the CaMKII/CREB/BDNF Pathway in Rats.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169