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CaMKII delta antibody

KO/KD Orthogonal
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Immunoprecipitation (IP). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401
Anti-CaMKII delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401
Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in (). GTX111401
Anti-CaMKII delta antibody used in (). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

Cat. No. GTX111401

Reference 24
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit
Package
100 μl ($399),
25 μl ($169)
See all CaMKII delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , 293T , A431 , HeLa , HepG2 , mouse brain , rat brain

Product Note

Cross-reactivity may occur with other CaM kinase II subunits.

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKII delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949809

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase II delta , CAMKD

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.

Database

Research Area

DATA IMAGES

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in Immunoprecipitation (IP). GTX111401

GTX111401 IP Image

Immunoprecipitation of CaMKII delta protein from HepG2 whole cell extracts using 5 μg of CaMKII delta antibody (GTX111401).
Western blot analysis was performed using CaMKII delta antibody (GTX111401).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

CaMK2D antibody detects CaMK2D protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
10% SDS-PAGE
CaMK2D antibody (GTX111401) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401

GTX111401 IHC-P Image

CaMKII delta antibody detects CaMKII delta protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CaMKII delta antibody (GTX111401) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CaMKII delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111401

GTX111401 ICC/IF Image

CaMKII delta antibody detects CaMKII delta protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CaMKII delta protein stained by CaMKII delta antibody (GTX111401) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401

GTX111401 IHC-P Image

CaMKII delta antibody detects CaMKII delta protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
CaMKII delta antibody (GTX111401) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401

GTX111401 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CaMKII delta antibody (GTX111401) at 1:300 dilution.

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:40000.

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

KO/KD

The data was published in the journal Nat Commun in 2019. PMID: 30962442

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

Orthogonal

The data was published in the journal J Cell Mol Med in 2018.PMID: 29266709

Anti-CaMKII delta antibody used in (). GTX111401

GTX111401 Image

The data was published in the journal Front Synaptic Neurosci in 2017.PMID: 29046633

Anti-CaMKII delta antibody used in (). GTX111401

GTX111401 Image

The data was published in the journal Front Synaptic Neurosci in 2017.PMID: 29046633

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

The data was published in the journal Cell Prolif in 2020.PMID: 32101357

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

The data was published in the journal Nat Commun in 2016.PMID: 27739424

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

The data was published in the journal Nat Commun in 2019. PMID: 30962442

REFERENCE

Application Reference

A Deb et al. JMCC PLUS 2024; 10 : 100093 Conditional ablation of MCU exacerbated cardiac pathology in a genetic arrhythmic model of CPVT
Liu SH et al. Int J Mol Sci 2024; 25 (14) Chronic Partial Sleep Deprivation Increased the Incidence of Atrial Fibrillation by Promoting Pulmonary Vein and Atrial Arrhythmogenesis in a Rodent Model.
Wei ZX et al. Cell Mol Life Sci 2024; 81 (1) : 88 Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling.
Lee TI et al. Int J Mol Sci 2023; 24 (22) Ketogenic Diet Regulates Cardiac Remodeling and Calcium Homeostasis in Diabetic Rat Cardiomyopathy.
Shiba N et al. Mol Ther Nucleic Acids 2023; 34 : 102060 Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.
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Submit a Reference

REVIEW

CaMKII delta antibody Cat. No. GTX111401

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Date : Anonymous submitted on 10-Dec-2012
Score :
Application Tested : WB
Sample Species : Rb
Sample : pulmonary vein tissue
Amount used : 7μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
A Deb et al. JMCC PLUS 2024; 10:100093 Conditional ablation of MCU exacerbated cardiac pathology in a genetic arrhythmic model of CPVT
Liu SH et al. Int J Mol Sci 2024; 25 (14) Chronic Partial Sleep Deprivation Increased the Incidence of Atrial Fibrillation by Promoting Pulmonary Vein and Atrial Arrhythmogenesis in a Rodent Model.
Wei ZX et al. Cell Mol Life Sci 2024; 81 (1):88 Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling.
Lee TI et al. Int J Mol Sci 2023; 24 (22) Ketogenic Diet Regulates Cardiac Remodeling and Calcium Homeostasis in Diabetic Rat Cardiomyopathy.
Shiba N et al. Mol Ther Nucleic Acids 2023; 34:102060 Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.
Rocco-Machado N et al. J Biol Chem 2022; 298 (11):102579 Oxidative stress-induced autonomous activation of the calcium/calmodulin-dependent kinase II involves disulfide formation in the regulatory domain.
Application : IP
Mao Zhang et al. Front Cardiovasc Med 2021; 8:820416 CaMKII-δ9 Induces Cardiomyocyte Death to Promote Cardiomyopathy and Heart Failure.
Application : WB
Reactivity : Mouse
Lee TW et al. Int J Mol Sci 2021; 23 (1) Fibroblast Growth Factor 23 Stimulates Cardiac Fibroblast Activity through Phospholipase C-Mediated Calcium Signaling.
Application : WB
Reactivity : Human
Zhong G et al. Front Cell Dev Biol 2021; 9:739944 WWP1 Deficiency Alleviates Cardiac Remodeling Induced by Simulated Microgravity.
Application : WB
Reactivity : Mouse
Joseph LC et al. Sci Rep 2021; 11 (1):17808 The mitochondrial calcium uniporter promotes arrhythmias caused by high-fat diet.
Application : WB
Reactivity : Mouse
Himelman E et al. J Clin Invest 2020; 130 (4):1713-1727 Prevention of connexin-43 remodeling protects against Duchenne muscular dystrophy cardiomyopathy.
Hung Y et al. Europace 2020; 22 (7):1132-1141 Klotho modulates electrical activity and calcium homeostasis in pulmonary vein cardiomyocytes via PI3K/Akt signalling.
Application : WB
Reactivity : Rabbit
Liu C et al. Cell Prolif 2020; 53 (3):e12783 Alteration of calcium signalling in cardiomyocyte induced by simulated microgravity and hypergravity.
Application : WB
Reactivity : Mouse
Zhang M et al. Nat Cell Biol 2019; 21 (9):1152-1163 CaMKII-帤9 promotes cardiomyopathy through disrupting UBE2T-dependent DNA repair.
Application : WB
Reactivity : Human
Cruz Junho CV et al. Braz J Med Biol Res 2019; 52 (7):e8732 Ca2+/Calmodulin-dependent kinase II delta B is essential for cardiomyocyte hypertrophy and complement gene expression after LPS and HSP60 stimulation in vitro.
Application : WB
Li J et al. Nat Commun 2019; 10 (1):1589 TMCO1-mediated Ca2+ leak underlies osteoblast functions via CaMKII signaling.
Application : WB
Reactivity : Mouse
Li S et al. Cell Physiol Biochem 2018; 46 (3):1042-1054 CaMKII Potentiates Store-Operated Ca2+ Entry Through Enhancing STIM1 Aggregation and Interaction with Orai1.
Application : WB
Reactivity : Human
Meese S et al. Front Synaptic Neurosci 2017; 9:13 Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin.
Jin L et al. J Cell Mol Med 2017; (Epub) Gallic acid attenuates calcium calmodulin-dependent kinase II-induced apoptosis in spontaneously hypertensive rats.
Application : WB
Reactivity : Rat
Xu S et al. Nat Commun 2016; 7:13189 CaMKII induces permeability transition through Drp1 phosphorylation during chronic β-AR stimulation.
Application : WB
Morita T et al. J Vet Med Sci 2014; 76 (7):963-9 Mechanisms underlying a decrease in KCl-induced contraction after long-term serum-free organ culture of rat isolated mesenteric artery.
Application : WB
Reactivity : Rat
Hayashi T et al. FEBS J 2014; Post-translational dual regulation of cytochrome-P450 aromatase at the catalytic and protein levels by phosphorylation/dephosphorylation.
Application : WB
Reactivity : Human
Kao YH et al. Eur J Clin Invest 2014; FGF-23 Dysregulates Calcium Homeostasis and Electrophysiological Properties in HL-1 Atrial Cells.
Application : WB
Reactivity : Mouse
Kao YH et al. Int J Cardiol 2013; 168 (4):4178-83 Histone deacetylase inhibition improved cardiac functions with direct antifibrotic activity in heart failure.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169