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CaMKII delta antibody

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Immunoprecipitation (IP). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111401
Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401
Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401
Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401

Cat No. GTX111401

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Rabbit
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , mouse brain , rat brain

Specificity/Sensitivity

Cross-reactivity may occur with other CaM kinase II subunits.

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CaMKII delta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calcium/calmodulin dependent protein kinase II delta , CAMKD

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.

Database

Research Area

DATA IMAGES

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in Immunoprecipitation (IP). GTX111401

GTX111401 IP Image

Immunoprecipitation of CaMKII delta protein from HepG2 whole cell extracts using 5 μg of CaMKII delta antibody (GTX111401).
Western blot analysis was performed using CaMKII delta antibody (GTX111401).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

CaMK2D antibody detects CaMK2D protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HCT116 whole cell lysate/extract
10% SDS-PAGE
CaMK2D antibody (GTX111401) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111401

GTX111401 ICC/IF Image

CaMKII delta antibody detects CaMKII delta protein at cytoplasm by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CaMKII delta protein stained by CaMKII delta antibody (GTX111401) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-CaMKII delta antibody used in Western Blot (WB). GTX111401

GTX111401 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody (GTX111401) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401

GTX111401 IHC-P Image

CaMKII delta antibody detects CaMKII delta protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
CaMKII delta antibody (GTX111401) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CaMKII delta antibody used in IHC (Paraffin sections) (IHC-P). GTX111401

GTX111401 IHC-P Image

CaMKII delta antibody detects CaMKII delta protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CaMKII delta antibody (GTX111401) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

CaMKII delta antibody Cat No. GTX111401

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Dec-2012
Score :
Application Tested : WB
Sample Species : Rb
Sample : pulmonary vein tissue
Amount used : 7μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169