For WB, use at a dilution of 1:1,000, For IF, use at a dilution of 1:50 with paraffin-embedded sections and 1:100 with frozen sections.
Mouse. Predicted to react with human based on 100% sequence identity.
This product is supplied as unpurified antiserum containing 0.05% NaN3.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
CaMKII synthetic peptide with oxidized Met281/282
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CAMK, CAMK-II, CAMKG
Calcium/calmodulin-dependent protein kinase II (CaMKII) couples increases in Ca2+ to fundamental responses in excitable cells including activation of ion channels, gene transcription and apoptosis. CaMKII is activated by angiotensin II-induced oxidation, which leads to apoptosis in cardiomyocytes. CaMKII activity is also enhanced by pro-oxidant conditions, and the oxidation of paired regulatory domain methionine residues sustains CaMKII activity in the absence of Ca2+/CaM.