WB analysis of various sample lysates using using GTX36254 CaMKII (oxidized) antibody.
Lane 1 : Recombinant CaMKII
Lane 2 : Untreated mouse heart cell lysates
Lane 3 : H2O2-treated mouse heart cell lysates
Dilution : 1:1000

Immunoblot of WT CaMKII and M281/282V mutant after no treatment, oxidation, or autophosphorylation probed with antibodies against total, autophosphorylated, or oxidized M281/282 CaMKII (GTX36254).

Immunoblot of oxidized WT CaMKII probed with antiserum against oxidized M281/282 with increasing ratios of oxidized peptide.

Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized (GTX36254) or total CaMKII and blue staining for nuclei.

Immunofluorescent staining of heart sections from mice treated with saline, AngII, or Isoproterenol and probed for oxidized or total CaMKII. Red staining is positive for oxidized or total CaMKII, and blue staining is positive for nuclei. Mice lacking a critical subunit of NADPH oxidase (p47-/-) did not show increased levels of oxidized CaMKII in response to AngII.