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CaMKII (oxidized) antibody

KO/KD Orthogonal Overexpression
Anti-CaMKII (oxidized) antibody used in Western Blot (WB). GTX36254
Anti-CaMKII (oxidized) antibody used in IHC (Paraffin sections) (IHC-P). GTX36254
Anti-CaMKII (oxidized) antibody used in IHC (Paraffin sections) (IHC-P). GTX36254
Anti-CaMKII (oxidized) antibody used in Western Blot (WB). GTX36254
Anti-CaMKII (oxidized) antibody used in Western Blot (WB). GTX36254

Cat. No. GTX36254

Reference 31

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, PLA

Reactivity

 Human, Mouse, Rat
Package
50 μl ($399)
See all CaMKII products

APPLICATION

Application Note

For WB, use at a dilution of 1:1,000, For IF, use at a dilution of 1:50 with paraffin-embedded sections and 1:100 with frozen sections.

Product Note

Mouse. Predicted to react with human based on 100% sequence identity.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

CaMKII synthetic peptide with oxidized Met281/282

Conjugation

Unconjugated

RRID

AB_2887751

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAMK , CAMK-II , CAMKG

Background

Calcium/calmodulin-dependent protein kinase II (CaMKII) couples increases in Ca2+ to fundamental responses in excitable cells including activation of ion channels, gene transcription and apoptosis. CaMKII is activated by angiotensin II-induced oxidation, which leads to apoptosis in cardiomyocytes. CaMKII activity is also enhanced by pro-oxidant conditions, and the oxidation of paired regulatory domain methionine residues sustains CaMKII activity in the absence of Ca2+/CaM.

Research Area

DATA IMAGES

Anti-CaMKII (oxidized) antibody used in Western Blot (WB). GTX36254

GTX36254 WB Image

WB analysis of various sample lysates using using GTX36254 CaMKII (oxidized) antibody.
Lane 1 : Recombinant CaMKII
Lane 2 : Untreated mouse heart cell lysates
Lane 3 : H2O2-treated mouse heart cell lysates
Dilution : 1:1000

Anti-CaMKII (oxidized) antibody used in IHC (Paraffin sections) (IHC-P). GTX36254

GTX36254 IHC-P Image

Immunofluorescent staining of heart sections from mice treated with saline, AngII, or Isoproterenol and probed for oxidized or total CaMKII. Red staining is positive for oxidized or total CaMKII, and blue staining is positive for nuclei. Mice lacking a critical subunit of NADPH oxidase (p47-/-) did not show increased levels of oxidized CaMKII in response to AngII.

Anti-CaMKII (oxidized) antibody used in IHC (Paraffin sections) (IHC-P). GTX36254

GTX36254 IHC-P Image

Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized (GTX36254) or total CaMKII and blue staining for nuclei.

Anti-CaMKII (oxidized) antibody used in Western Blot (WB). GTX36254

GTX36254 WB Image

Immunoblot of oxidized WT CaMKII probed with antiserum against oxidized M281/282 with increasing ratios of oxidized peptide.

Anti-CaMKII (oxidized) antibody used in Western Blot (WB). GTX36254

GTX36254 WB Image

Immunoblot of WT CaMKII and M281/282V mutant after no treatment, oxidation, or autophosphorylation probed with antibodies against total, autophosphorylated, or oxidized M281/282 CaMKII (GTX36254).

REFERENCE

Application Reference

? Kov?cs et al. Geroscience 2024; Sex-specific cardiovascular remodeling leads to a divergent sex-dependent development of heart failure in aged hypertensive rats
Application : WB
Wei ZX et al. Cell Mol Life Sci 2024; 81 (1) : 88 Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling.
F Li et al. Eur J Pharmacol 2023; 961 : 176167 Glucose fluctuations aggravated the late sodium current induced ventricular arrhythmias via the activation of ROS/CaMKII pathway
Lu S et al. Int J Mol Sci 2023; 24 (18) Stachydrine Hydrochloride Regulates the NOX2-ROS-Signaling Axis in Pressure-Overload-Induced Heart Failure.
Zhao J et al. Redox Biol 2023; 59 : 102594 Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress.
Application : WB
Reactivity : Mouse
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REVIEW

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Application Reference
? Kov?cs et al. Geroscience 2024; Sex-specific cardiovascular remodeling leads to a divergent sex-dependent development of heart failure in aged hypertensive rats
Application : WB
Wei ZX et al. Cell Mol Life Sci 2024; 81 (1):88 Zbtb16 increases susceptibility of atrial fibrillation in type 2 diabetic mice via Txnip-Trx2 signaling.
F Li et al. Eur J Pharmacol 2023; 961:176167 Glucose fluctuations aggravated the late sodium current induced ventricular arrhythmias via the activation of ROS/CaMKII pathway
Lu S et al. Int J Mol Sci 2023; 24 (18) Stachydrine Hydrochloride Regulates the NOX2-ROS-Signaling Axis in Pressure-Overload-Induced Heart Failure.
Zhao J et al. Redox Biol 2023; 59:102594 Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress.
Application : WB
Reactivity : Mouse
Hu Q et al. Alcohol Clin Exp Res 2022; 46 (5):707-723 Impact and potential mechanism of effects of chronic moderate alcohol consumption on cardiac function in aldehyde dehydrogenase 2 gene heterozygous mice.
Application : WB
Reactivity : Mouse
Xian Luo et al. Oxid Med Cell Longev 2022; 2022:8538296 Ageing Increases Cardiac Electrical Remodelling in Rats and Mice via NOX4/ROS/CaMKII-Mediated Calcium Signalling.
Application : WB
Julian Mustroph et al. Front Immunol 2021; 12:740260 Cardiac Fibrosis Is a Risk Factor for Severe COVID-19.
Application : WB
Reactivity : Human
Joseph LC et al. Sci Rep 2021; 11 (1):17808 The mitochondrial calcium uniporter promotes arrhythmias caused by high-fat diet.
Application : WB
Reactivity : Mouse
Jiang H et al. Oxid Med Cell Longev 2021; 2021:6673967 Tilianin Ameliorates Cognitive Dysfunction and Neuronal Damage in Rats with Vascular Dementia via p-CaMKII/ERK/CREB and ox-CaMKII-Dependent MAPK/NF-κB Pathways.
Application : WB, ICC/IF
Reactivity : Human
Woo M et al. Transl Clin Pharmacol 2021; 29 (2):92-106 Insulin sensitization causes accelerated sinus nodal dysfunction through autophagic dysregulation in hypertensive mice.
Pradhan K et al. J Immunol 2021; TRAM-Related TLR4 Pathway Antagonized by IRAK-M Mediates the Expression of Adhesion/Coactivating Molecules on Low-Grade Inflammatory Monocytes.
Calum J McMullen et al. Front Cardiovasc Med 2020; 7:630480 Sunitinib and Imatinib Display Differential Cardiotoxicity in Adult Rat Cardiac Fibroblasts That Involves a Role for Calcium/Calmodulin Dependent Protein Kinase II.
Application : WB
Reactivity : Human
Jiang H et al. Biomed Res Int 2020; 2020:5939715 Tilianin Protects against Ischemia/Reperfusion-Induced Myocardial Injury through the Inhibition of the Ca2+/Calmodulin-Dependent Protein Kinase II-Dependent Apoptotic and Inflammatory Signaling Pathways.
Application : WB
Reactivity : Rat
Yang X et al. Redox Biol 2020; 30:101432 Enhanced cardiomyocyte reactive oxygen species signaling promotes ibrutinib-induced atrial fibrillation.
Chen HH et al. J Ethnopharmacol 2019; 248:112306 Stachydrine hydrochloride alleviates pressure overload-induced heart failure and calcium mishandling on mice.
Application : WB
Reactivity : Mouse
Shi T et al. Neurobiol Learn Mem 2019; 166:107104 Loss of MsrB1 perturbs spatial learning and long-term potentiation/long-term depression in mice.
Application : WB
Reactivity : Mouse
Chen WJ et al. J Mol Cell Cardiol 2019; 135:67-78 Tachycardia-induced CD44/NOX4 signaling is involved in the development of atrial remodeling.
Application : WB
Reactivity : Mouse
McCluskey C et al. Vascul Pharmacol 2019; (Epub) Compromised cardiovascular function in aged rats corresponds with increased expression and activity of calcium/calmodulin dependent protein kinase IIδ in aortic endothelium.
Application : WB
Reactivity : Rat
Miller BA et al. J Cell Physiol 2019; (Epub) Trpm2 enhances physiological bioenergetics and protects against pathological oxidative cardiac injury: Role of Pyk2 phosphorylation.
Application : WB
Reactivity : Mouse
Jiang H et al. Life Sci 2019; 216:233-245 Tilianin mediates neuroprotection against ischemic injury by attenuating CaMKII-dependent mitochondrion-mediated apoptosis and MAPK/NF-κB signaling.
Application : ICC/IF
Reactivity : Human
Montnach J et al. J Mol Cell Cardiol 2018; 123:13-25 Arrhythmias precede cardiomyopathy and remodeling of Ca2+ handling proteins in a novel model of long QT syndrome.
Application : WB
Reactivity : Mouse
Martin TP et al. Cell Signal 2018; 51:166-175 CaMKIIδ interacts directly with IKKβ and modulates NF-κB signalling in adult cardiac fibroblasts.
Application : PLA
Reactivity : Rat
Dewenter M et al. Circ Heart Fail 2017; 10 (5):e003840 Calcium/Calmodulin-Dependent Protein Kinase II Activity Persists During Chronic β-Adrenoceptor Blockade in Experimental and Human Heart Failure.
Application : WB
Reactivity : Mouse
Maity B et al. Cancer Res 2017; (Epub) Atypical G protein β5 promotes cardiac oxidative stress, apoptosis, and fibrotic remodeling in response to multiple cancer chemotherapeutics.
Application : WB
Reactivity : Mouse
Zuo S et al. Europace 2017; (Epub) Acute administration of tumour necrosis factor-α induces spontaneous calcium release via the reactive oxygen species pathway in atrial myocytes.
Application : WB
Reactivity : Mouse
Ingoglia G et al. Free Radic Biol Med 2017; (Epub) Hemopexin counteracts systolic dysfunction induced by heme-driven oxidative stress.
Application : WB
Reactivity : Mouse
Yeh YH et al. Basic Res Cardiol 2016; 111 (5):58 Protective role of heme oxygenase-1 in atrial remodeling.
Application : WB
Reactivity : Mouse
Popescu I et al. Cardiovasc Res 2016; (Epub) Elevated local [Ca2+] and CaMKII promote spontaneous Ca2+ release in ankyrin-B-deficient hearts.
Application : WB
Reactivity : Mouse
An M et al. Anat Cell Biol 2015; 48 (4):235-43 Protective effects of kaempferol against cardiac sinus node dysfunction via CaMKII deoxidization.
Application : WB
Reactivity : Mouse
Erickson JR et al. Cell 2008; 133 (3):462-74 A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation.
Application : WB
Reactivity : Mouse
Package List Price ($)
$ 399