CaMKII (phospho Thr286) antibody [HL3662]

Specificity test was performed by coating the plate with recombinant CaMKII alpha non-phospho peptide, CaMKII alpha (phospho Thr286) peptide, CaMKII beta non-phospho peptide, CaMKII beta (phospho Thr287) peptide, CaMKII delta non-phospho peptide, CaMKII delta (phospho Thr287) peptide, CaMKII gamma non-phospho peptide, and CaMKII gamma (phospho Thr287) peptide (250 ng/mL). Coated protein was probed with CaMKII (phospho Thr286) antibody [HL3662] (GTX641653) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Untreated (–) and treated (+) MKN-45 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CaMKII (phospho Thr286) antibody [HL3662] (GTX641653) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.