GeneTex
United States (US)

CaMKIV antibody [N1C3]

GTX106023_39974_20160922_WB_M_R_19011506_806.jpg
GTX106023_WB_19011506_467.jpg
GTX106023_39974_IHC_19011506_837.jpg

Cat No. GTX106023

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.92mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CAMK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CaMK IV antibody, CaMK-GR antibody, IV antibody, MGC36771 antibody, caMK antibody, CAMK4 antibody, CAM kinase- GR antibody, caM kinase-GR antibody, brain Ca++-calmodulin-dependent protein kinase type IV antibody, calcium/calmodulin-dependent protein kinase type IV antibody, calcium/calmodulin-dependent protein kinase type IV catalytic chain antibody, brain Ca(2+)-calmodulin-dependent protein kinase type IV antibody, CAM kinase IV antibody, calcium/calmodulin-dependent protein kinase IV antibody

Background

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106023_39974_20160922_WB_M_R_19011506_806.jpg

GTX106023 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CAMK4 antibody [N1C3] (GTX106023) diluted at 1:500.

GTX106023_WB_19011506_467.jpg

GTX106023 WB Image

Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
GTX106023 diluted at 1:2000

GTX106023_39974_IHC_19011506_837.jpg

GTX106023 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CaMKIV(GTX106023) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf