Calbindin + Calretinin antibody [HL1787]

Calbindin + Calretinin antibody [HL1787] detects Calbindin + Calretinin protein at cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: Calbindin + Calretinin stained by Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Calbindin antibody [HL1787] detects Calbindin protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.
Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.
Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min