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Calbindin + Calretinin antibody [HL1787]

Recombinant antibody Overexpression
Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439
Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439
Anti-Calbindin + Calretinin antibody [HL1787] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637439
Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439
Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Frozen sections) (IHC-Fr). GTX637439
Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439
Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439
Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439

Cat. No. GTX637439

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1787

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calbindin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Calbindin

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calbindin 1 , CALB , D-28K

Cellular Localization

Nucleus,Cytosol

Background

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439

GTX637439 IHC-P Image

Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.
Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Paraffin sections) (IHC-P). GTX637439

GTX637439 IHC-P Image

Calbindin antibody [HL1787] detects Calbindin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.
Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin + Calretinin antibody [HL1787] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637439

GTX637439 ICC/IF Image

Calbindin antibody [HL1787] detects Calbindin protein at cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calbindin stained by Calbindin antibody [HL1787] (GTX637439) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439

GTX637439 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calbindin + Calretinin antibody [HL1787] used in IHC (Frozen sections) (IHC-Fr). GTX637439

GTX637439 IHC-Fr Image

Calbindin + Calretinin antibody [HL1787] detects Calbindin + Calretinin protein at cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: Calbindin + Calretinin stained by Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439

GTX637439 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439

GTX637439 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calbindin + Calretinin antibody [HL1787] used in Western Blot (WB). GTX637439

GTX637439 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (GTX637439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations