For IHC-P: Use at a dilution of 1:150-1:300 in ABC method. An incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 or enzymatic digestion prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user.
Antibody reacts specifically with calbindin-D-28K in brain and kidney tissues from human, cow, goat, pig, rabbit, dog, cat, rat, mouse, sheep, and guinea pig sources. It binds weakly to chicken calbindin-D-28K.
Ascites containing 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Purified bovine kidney calbindin-D-28K
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and is related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. This Calbindin antibody is often used as a marker for cerebellum Purkinje cells.