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Calbindin antibody [GT386]

Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833
Anti-Calbindin antibody [GT386] used in Western Blot (WB). GTX634833
Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833
Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

Cat. No. GTX634833

Reference 1
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT386

Isotype

 IgG2a

Application

 WB, IHC-P, IHC (Free Floating)

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calbindin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Predict Reactivity

Human, Rabbit, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Calbindin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888488

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calbindin 1 , CALB , D-28K

Background

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

GTX634833 IHC-P Image

Calbindin antibody [GT386] detects Calbindin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: Calbindin stained by Calbindin antibody [GT386] (GTX634833) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody [GT386] used in Western Blot (WB). GTX634833

GTX634833 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [GT386] (GTX634833) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

GTX634833 IHC-P Image

Calbindin antibody [GT386] detects Calbindin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Calbindin stained by Calbindin antibody [GT386] (GTX634833) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833

GTX634833 IHC-P Image

Calbindin antibody [GT386] detects Calbindin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Calbindin stained by Calbindin antibody [GT386] (GTX634833) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Tsai CW et al. Neurotherapeutics 2022; 19 (2) : 649-659 Transcranial Ultrasound Stimulation Reverses Behavior Changes and the Expression of Calcium-Binding Protein in a Rodent Model of Schizophrenia.
Submit a Reference

REVIEW

Calbindin antibody [GT386] Cat. No. GTX634833

Rating
( Average 5 based on 3 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
IHC (Free Floating)(IHC (Free Floating))
( Average 5 based on 2 users reviews)
Anti-Calbindin antibody [GT386] used in IHC (Paraffin sections) (IHC-P). GTX634833
Date : Estela Maris Muñoz submitted on 08-Apr-2022
Score :
Application Tested : IHC-P
Sample Species : Rat
Sample : Rat cerebellum tissue section
Blocking : 10% Normal Donkey Serum Buffer, 25°C, 1Hr
Fixation : 4% PFA in PBS, 4°C, > 12Hr
Permeabilization /Antigen retrieval : 10 mM citrate buffer (pH6), 0.5Hr
Primary Antibody : 1:500 diluted in PBS with 2% NDSB and 1% Triton X-100, 4°C, 16Hr
Figure Note : IHC and confocal microscopy for a calbindin-positive Purkinje cell present in the cerebellum of an adult male Wistar rat.
A1-A2: Immunolabeling for calbindin (Calb; red). Nuclei (Nu) were counterstained with DAPI (grey). Purkinje neurons (PkC) express calbindin in their somas and projections. These GABAergic cells exhibit an intricately elaborate dendritic arbor towards the cerebellar molecular layer (yellow arrow) and a single long axon (cyan arrow) that forms synapsis in the deep cerebellar nuclei.
A3: 3D reconstruction of the main projections of the Purkinje cell pointed in A and B. Image generated at 60X with 2.0 zoom, in an Olympus FV1000 confocal microscope.
Authors: Martin Avila and Estela Muñoz (IHEM-UNCuyo-CONICET, Mendoza, Argentina).
Anti-Calbindin antibody [GT386] used in IHC (Free Floating) (IHC (Free Floating)). GTX634833
Date : Anonymous submitted on 23-Jun-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : Mouse Brain cerebellum 250μm slice
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody : 1:100, 4°C, 48Hr
Figure Note : Calbindin (red) & VGLUT2 (green, GTX133142)
Anti-Calbindin antibody [GT386] used in IHC (Free Floating) (IHC (Free Floating)). GTX634833
Date : Wei-Hao Liao submitted on 04-Jun-2021
Score :
Application Tested : IHC (Free Floating)
Sample Species : Ms
Sample : Mouse Brain cerebellum 250μm slice
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody : 1:100, 4°C, 48Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399
$ 169