APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human CALB1 protein, and it does not cross react with human CALB2 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Calbindin.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calbindin 1 , CALB , D-28K
Cellular Localization
Nucleus,Cytoplasm, cytosol
Background
The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
Database
Research Area
DATA IMAGES
![Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587](/templates/default/js/lightbox/images/loading.gif "Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587")
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GTX638587 IHC-P Image
Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: Calbindin stained by Calbindin antibody [HL2379] (GTX638587) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638587 IHC-P Image
Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: Calbindin stained by Calbindin antibody [HL2379] (GTX638587) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638587 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638587 ICC/IF Image
Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calbindin stained by Calbindin antibody [HL2379] (GTX638587) diluted at 1:250.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638587 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638587 WB Image
Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638587 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
There are currently no references for Calbindin antibody [HL2379] (GTX638587). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Calbindin antibody [HL2379] (GTX638587). Be the first to share your experience with this product.
![Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587](/upload/website/prouct_img/small/GTX638587/GTX638587_T-45040_20230526_IHC-P_M_1_23060622_806.webp "Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587")
![Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587](/upload/website/prouct_img/small/GTX638587/GTX638587_T-45040_20230526_IHC-P_R_23060622_596.webp "Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/small/GTX638587/GTX638587_T-45040_20230519_WB_multiple_B_23053001_415.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638587](/upload/website/prouct_img/small/GTX638587/GTX638587_T-45040_20230519_ICC_IF_R_23053001_278.webp "Anti-Calbindin antibody [HL2379] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/small/GTX638587/GTX638587_T-45040_20230519_WB_M_R_23053001_730.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/small/GTX638587/GTX638587_T-45040_20230519_WB_brain_23053001_426.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/small/GTX638587/GTX638587_45110_20230721_WB_TPM_watermark_23072519_673.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")
![Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587](/upload/website/prouct_img/normal/GTX638587/GTX638587_T-45040_20230526_IHC-P_M_1_23060622_806.webp "Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587")
![Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587](/upload/website/prouct_img/normal/GTX638587/GTX638587_T-45040_20230526_IHC-P_R_23060622_596.webp "Anti-Calbindin antibody [HL2379] used in IHC (Paraffin sections) (IHC-P). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/normal/GTX638587/GTX638587_T-45040_20230519_WB_multiple_B_23053001_415.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638587](/upload/website/prouct_img/normal/GTX638587/GTX638587_T-45040_20230519_ICC_IF_R_23053001_278.webp "Anti-Calbindin antibody [HL2379] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/normal/GTX638587/GTX638587_T-45040_20230519_WB_M_R_23053001_730.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/normal/GTX638587/GTX638587_T-45040_20230519_WB_brain_23053001_426.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")
![Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587](/upload/website/prouct_img/normal/GTX638587/GTX638587_45110_20230721_WB_TPM_watermark_23072519_673.webp "Anti-Calbindin antibody [HL2379] used in Western Blot (WB). GTX638587")