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Calbindin antibody

Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856
Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856
Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in Western Blot (WB). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856
Anti-Calbindin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130856
Anti-Calbindin antibody used in Western Blot (WB). GTX130856
Anti-Calbindin antibody used in Western Blot (WB). GTX130856

Cat No. GTX130856

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of Human Calbindin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

calbindin 1 , CALB , D-28K

Background

Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856

GTX130856 IHC-Fr Image

Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856

GTX130856 IHC-Fr Image

Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin antibody used in IHC (Frozen sections) (IHC-Fr). GTX130856

GTX130856 IHC-Fr Image

Calbindin antibody detects Calbindin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: Calbindin protein stained by Calbindin antibody (GTX133218) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Calbindin antibody (GTX130856) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in Western Blot (WB). GTX130856

GTX130856 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CALB1antibody (GTX130856) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: Calbindin protein stained by Calbindin antibody (GTX130856) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in IHC (Paraffin sections) (IHC-P). GTX130856

GTX130856 IHC-P Image

Calbindin antibody detects Calbindin protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Calbindin antibody (GTX130856) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calbindin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130856

GTX130856 ICC/IF Image

Calbindin antibody detects Calbindin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calbindin stained by Calbindin antibody (GTX130856) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calbindin antibody used in Western Blot (WB). GTX130856

GTX130856 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with Calbindin antibody (GTX130856) diluted at 1:1000 and competitor's antibody (sc-7691) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Calbindin antibody used in Western Blot (WB). GTX130856

GTX130856 WB Image

IMR-32 whole cell extracts (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody (GTX130856) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169