GeneTex
United States (US)

Calcineurin A antibody

GTX105003_41087_IFA_19011506_235.jpg
GTX105003_41087_20170908_WB_M_brain_19011506_551.jpg
GTX105003_41087_20170908_WB_R_brain_19011506_136.jpg

Cat No. GTX105003

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calcineurin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PPP3CA Antibody, CCN1 Antibody, protein phosphatase 3, catalytic subunit, alpha isozyme Antibody, PPP2B Antibody, CNA1 Antibody, CALNA1 Antibody, CALN Antibody, CALNA Antibody

Cellular Localization

Nucleus (By similarity)

Database

Research Area

DATA IMAGES
GTX105003_41087_IFA_19011506_235.jpg

GTX105003 ICC/IF Image

Calcineurin A antibody detects Calcineurin A protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calcineurin A protein stained by Calcineurin A antibody (GTX105003) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105003_41087_20170908_WB_M_brain_19011506_551.jpg

GTX105003 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX105003) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105003_41087_20170908_WB_R_brain_19011506_136.jpg

GTX105003 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX105003) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169