GeneTex
United States (US)

Calcineurin A antibody

GTX111039_40471_WB_2_19011507_941.jpg
GTX111039_40471_20141212_WB_R_brain_19011507_599.jpg
GTX111039_40471_20150508_WB_B_19011507_563.jpg
GTX111039_40471_20141212_WB_M_19011507_197.jpg
GTX111039_40471_20141212_WB_19011507_736.jpg
GTX111039_40471_20180131_ICC_IF_R_19011507_254.jpg

Cat No. GTX111039

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat, Frog, Squirrel
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Molt-4 , U87-MG , SK-N-SH , SK-N-AS , IMR32 , JC , BCL-1 , rat brain , human Calcineurin A-transfected 293T

Predict Reactivity

Mouse, Zebrafish, Rabbit, Bovine, Dog, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calcineurin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Phosphatase 3 Catalytic Subunit Alpha,Caln,Calna,Calna1,Ccn1,Cna1,Iecee,Iecee1,Ppp2B,Ppp3Ca

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
GTX111039_40471_WB_2_19011507_941.jpg

GTX111039 WB Image

Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000

GTX111039_40471_20141212_WB_R_brain_19011507_599.jpg

GTX111039 WB Image

Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000.

GTX111039_40471_20150508_WB_B_19011507_563.jpg

GTX111039 WB Image

Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Non-transfected (-) and Calcineurin A-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) at a dilution of 1:5000.

GTX111039_40471_20141212_WB_M_19011507_197.jpg

GTX111039 WB Image

Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000.

GTX111039_40471_20141212_WB_19011507_736.jpg

GTX111039 WB Image

Calcineurin A antibody detects Calcineurin A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Calcineurin A antibody (GTX111039) diluted by 1:3000.

GTX111039_40471_20180131_ICC_IF_R_19011507_254.jpg

GTX111039 ICC/IF Image

Calcineurin A antibody detects Calcineurin A protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calcineurin A stained by Calcineurin A antibody (GTX111039) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169