Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.