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Calcineurin B antibody [HL2419]

Recombinant antibody Overexpression
Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641
Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641
Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641
Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641
Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641
Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641

Cat. No. GTX638641

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2419

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calcineurin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Calcineurin B

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 3 regulatory subunit B, alpha , CALNB1 , CNB , CNB1

Cellular Localization

Cytoplasm, cytosol,Cell membrane

Background

Enables cyclosporin A binding activity; phosphatase binding activity; and protein domain specific binding activity. Involved in calcineurin-NFAT signaling cascade and positive regulation of transcription by RNA polymerase II. Part of calcineurin complex. Implicated in Alzheimer's disease and dilated cardiomyopathy. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641

GTX638641 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641

GTX638641 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641

GTX638641 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641

GTX638641 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641

GTX638641 WB Image

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcineurin B antibody [HL2419] used in Western Blot (WB). GTX638641

GTX638641 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations