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Calcineurin B antibody

Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181
Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181
Anti-Calcineurin B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114181
Anti-Calcineurin B antibody used in IHC (Paraffin sections) (IHC-P). GTX114181
Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181
Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181
Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181

Cat. No. GTX114181

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

IMR32 , mouse fetal brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calcineurin B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163374

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 3 regulatory subunit B, alpha , CALNB1 , CNB , CNB1

Background

Enables cyclosporin A binding activity; phosphatase binding activity; and protein domain specific binding activity. Involved in calcineurin-NFAT signaling cascade and positive regulation of transcription by RNA polymerase II. Part of calcineurin complex. Implicated in Alzheimer's disease and dilated cardiomyopathy. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181

GTX114181 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:50000.

Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181

GTX114181 WB Image

Sample (30 ug of whole cell lysate)

A: IMR32

12% SDS PAGE

GTX114181 diluted at 1:1000

Anti-Calcineurin B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114181

GTX114181 ICC/IF Image

Calcineurin B antibody detects Calcineurin B protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calcineurin B stained by Calcineurin B antibody (GTX114181) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calcineurin B antibody used in IHC (Paraffin sections) (IHC-P). GTX114181

GTX114181 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Calcineurin B(GTX114181) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181

GTX114181 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:1000.

Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181

GTX114181 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcineurin B antibody used in Western Blot (WB). GTX114181

GTX114181 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (GTX114181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

Calcineurin B antibody Cat. No. GTX114181

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Jun-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : MEF cells
Amount used : 40μg
Blocking : 5% non-fat milk, 22°C, 1Hr
Primary Antibody : 1:800, 4°C, 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169