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Calcium Sensing Receptor antibody [5C10, ADD]

Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Frozen sections) (IHC-Fr). GTX19347
Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Paraffin sections) (IHC-P). GTX19347
Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Paraffin sections) (IHC-P). GTX19347
Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in Western Blot (WB). GTX19347
Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Frozen sections) (IHC-Fr). GTX19347

Cat. No. GTX19347

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C10, ADD

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μg ($399)
See all Calcium Sensing Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 2 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues (214) A D D D Y G R P G I E K F RE E A E E R D I (235) of human calcium sensing receptor.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_423536

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcium sensing receptor , CAR , EIG8 , FHH , FIH , GPRC2A , HHC , HHC1 , HYPOC1 , NSHPT , PCAR1 , hCasR

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Frozen sections) (IHC-Fr). GTX19347

GTX19347 IHC-Fr Image

IHC-Fr analysis of mouse stomach tissue using GTX19347 Calcium Sensing Receptor antibody [5C10, ADD].
Green : Primary antibody
Blue : DAPI
Antigen retireval : sodium citrate buffer for 45 minutes at 4ºC, immersing in sodium citrate buffer for 10 minutes at 100ºC
Fixation : 4% formalin
Permeabilization : 0.3% Triton X-100 in PBS

Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Paraffin sections) (IHC-P). GTX19347

GTX19347 IHC-P Image

IHC-P analysis of human kidney tissue using GTX19347 Calcium Sensing Receptor antibody [5C10, ADD].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Paraffin sections) (IHC-P). GTX19347

GTX19347 IHC-P Image

IHC-P analysis of human brain tissue using GTX19347 Calcium Sensing Receptor antibody [5C10, ADD].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in Western Blot (WB). GTX19347

GTX19347 WB Image

WB analysis of HEK7-2 cell extract using GTX19347 Calcium Sensing Receptor antibody [5C10, ADD].

Anti-Calcium Sensing Receptor antibody [5C10, ADD] used in IHC (Frozen sections) (IHC-Fr). GTX19347

GTX19347 IHC-Fr Image

IHC-Fr analysis of bovine corneal epithelium (CE) limbus tissue using GTX19347 Calcium Sensing Receptor antibody [5C10, ADD].

REFERENCE

Application Reference

Wouter H van Megen et al. EBioMedicine 2022; 78 : 103947 Differential parathyroid and kidney Ca2+-sensing receptor activation in autosomal dominant hypocalcemia 1.
Application : WB
Reactivity : Mouse
Lee JJ et al. FASEB J 2021; 35 (11) : e21982 Activation of the calcium sensing receptor increases claudin-14 expression via a PLC -p38-Sp1 pathway.
Application : WB
Reactivity : Human
Oh IH et al. Am J Physiol Renal Physiol 2021; 320 (3) : F418-F428 Thick ascending limb claudins are altered to increase calciuria and magnesiuria in metabolic acidosis.
Lee JJ et al. JCI Insight 2019; 5 Activation of the calcium sensing receptor attenuates TRPV6-dependent intestinal calcium absorption.
Babiec WE et al. J Neurophysiol 2017; : jn.00628.2017 Novel Ca2+-Dependent Mechanisms Regulate Spontaneous Release at Excitatory Synapses onto CA1 Pyramidal Cells.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399