APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human Calcium Sensing Receptor Extracellular domain.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human Calcium Sensing Receptor. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium sensing receptor , CAR , EIG8 , FHH , FIH , GPRC2A , HHC , HHC1 , HYPOC1 , NSHPT , PCAR1 , hCasR
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a plasma membrane G protein-coupled receptor that senses small changes in circulating calcium concentration. The encoded protein couples this information to intracellular signaling pathways that modify parathyroid hormone secretion or renal cation handling, and thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene are a cause of familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/templates/default/js/lightbox/images/loading.gif "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
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GTX638563 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000 and competitor's antibody (Clone 5C10, ADD) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX638563 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638563 IHC-P Image
Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638563 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638563 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX638563 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638563 IHC-P Image
Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638563 IHC-P Image
Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (GTX638563) diluted at 1:400.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Calcium Sensing Receptor antibody [HL2357] (GTX638563). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Calcium Sensing Receptor antibody [HL2357] (GTX638563). Be the first to share your experience with this product.
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_45103_20240301_WB_competitor_watermark_24030600_300.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_45103_20230908_WB_C_23091319_428.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_T-45033_20230707_IHC-P_M_23071822_175.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_T-45033_20230512_WB_R_kidney_23051702_619.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_T-45033_20230512_WB_M_tissue_23051702_945.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_45103_20230714_WB_TPM_watermark_23071822_955.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_T-45033_20230707_IHC-P_R_23071822_814.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563](/upload/website/prouct_img/small/GTX638563/GTX638563_T-45033_20230707_IHC-P_23071822_932.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20240301_WB_competitor_watermark_24030600_300.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20230908_WB_C_23091319_428.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230707_IHC-P_M_23071822_175.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230512_WB_R_kidney_23051702_619.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230512_WB_M_tissue_23051702_945.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_45103_20230714_WB_TPM_watermark_23071822_955.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in Western Blot (WB). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230707_IHC-P_R_23071822_814.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563")
![Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563](/upload/website/prouct_img/normal/GTX638563/GTX638563_T-45033_20230707_IHC-P_23071822_932.webp "Anti-Calcium Sensing Receptor antibody [HL2357] used in IHC (Paraffin sections) (IHC-P). GTX638563")