APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse placenta, mouse pancreas, mouse kidney, unboiled TT
Predict Reactivity
Bovine, Cat, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.55 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human Calcium Sensing Receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949844
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium sensing receptor , CAR , EIG8 , FHH , FIH , GPRC2A , HHC , HHC1 , HYPOC1 , NSHPT , PCAR1 , hCasR
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101348 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calcium Sensing Receptor(GTX101348) antibody at 1:200 dilution.
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GTX101348 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Calcium Sensing Receptor(GTX101348) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101348 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101348 WB Image
Unboiled whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101348 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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