GeneTex
United States (US)

Calcium Sensing Receptor antibody

GTX101348_IHC_19011506_703.jpg
GTX101348_43061_20171215_WB_M_brain_19011506_863.jpg
GTX101348_40086_20170825_WB_M_placenta_19011506_592.jpg
GTX101348_WB_19011506_653.jpg
GTX101348_IFA_19011506_215.jpg

Cat No. GTX101348

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , mouse brain , mouse placenta

Predict Reactivity

Rat, Bovine, Cat, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calcium Sensing Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calcium Sensing Receptor,Car,Eig8,Fhh,Fih,Gprc2A,Hhc,Hhc1,Hypoc1,Nshpt,Pcar1,Hcasr,Casr

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101348_IHC_19011506_703.jpg

GTX101348 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Calcium Sensing Receptor(GTX101348) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101348_43061_20171215_WB_M_brain_19011506_863.jpg

GTX101348 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101348_40086_20170825_WB_M_placenta_19011506_592.jpg

GTX101348 WB Image

Mouse tissue extract (50 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101348_WB_19011506_653.jpg

GTX101348 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX101348 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101348_IFA_19011506_215.jpg

GTX101348 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Calcium Sensing Receptor(GTX101348) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169