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Calcium binding protein p22 antibody

Anti-Calcium binding protein p22 antibody used in Western Blot (WB). GTX113936
Anti-Calcium binding protein p22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113936
Anti-Calcium binding protein p22 antibody used in Western Blot (WB). GTX113936
Anti-Calcium binding protein p22 antibody used in IHC (Paraffin sections) (IHC-P). GTX113936
Anti-Calcium binding protein p22 antibody used in Western Blot (WB). GTX113936
Anti-Calcium binding protein p22 antibody used in IHC (Paraffin sections) (IHC-P). GTX113936

Cat. No. GTX113936

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calcium binding protein p22 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

mouse liver , rat liver , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calcium binding protein p22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036564

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calcineurin like EF-hand protein 1 , CHP , SLC9A1BP , Sid470p , p22 , p24

Cellular Localization

Cytoplasm

Background

This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calcium binding protein p22 antibody used in Western Blot (WB). GTX113936

GTX113936 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
B: HepG2 (GTX27900)
12% SDS PAGE
GTX113936 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcium binding protein p22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113936

GTX113936 ICC/IF Image

Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody (GTX113936) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Calcium binding protein p22 antibody used in Western Blot (WB). GTX113936

GTX113936 WB Image

Calcium binding protein P22 antibody detects CHP protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
12% SDS-PAGE
Calcium binding protein P22 antibody (GTX113936) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcium binding protein p22 antibody used in IHC (Paraffin sections) (IHC-P). GTX113936

GTX113936 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calcium binding protein P22(GTX113936) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calcium binding protein p22 antibody used in Western Blot (WB). GTX113936

GTX113936 WB Image

Calcium binding protein P22 antibody detects CHP protein by western blot analysis.
A. 50 μg rat liver lysate/extract
12% SDS-PAGE
Calcium binding protein P22 antibody (GTX113936) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calcium binding protein p22 antibody used in IHC (Paraffin sections) (IHC-P). GTX113936

GTX113936 IHC-P Image

Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Calcium binding protein P22 antibody (GTX113936) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Y Wu et al. Metabolites 2024; 14 (1) : 51 Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response
Application : WB
Reactivity : Human
Wang M et al. Cell Death Differ 2019; Trim32 suppresses cerebellar development and tumorigenesis by degrading Gli1/sonic hedgehog signaling.
Application : ICC/IF, IP
Reactivity : Mouse
Zhu XG et al. Mol Cell 2019; : pii: S1097-2765(19)30057-7 CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4.
Application : WB
Reactivity : Human, Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169