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Caldesmon antibody, Internal

Anti-Caldesmon antibody, Internal used in Western Blot (WB). GTX81812
Anti-Caldesmon antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81812
Anti-Caldesmon antibody, Internal used in Flow cytometry (FACS). GTX81812

Cat. No. GTX81812

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Caldesmon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 428-457 amino acids from the Central region of human CALD1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caldesmon 1 , CDM , H-CAD , HCAD , L-CAD , LCAD , NAG22

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Caldesmon antibody, Internal used in Western Blot (WB). GTX81812

GTX81812 WB Image

WB analysis of A2058 cell lysate (35ug/lane) using GTX81812 Caldesmon antibody, Internal.

Anti-Caldesmon antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81812

GTX81812 IHC-P Image

IHC-P analysis of human lung carcinoma using GTX81812 Caldesmon antibody, Internal.

Anti-Caldesmon antibody, Internal used in Flow cytometry (FACS). GTX81812

GTX81812 FACS Image

FACS analysis of NCI-H292 cells using GTX81812 Caldesmon antibody, Internal.
Top histogram : negative control
Bottom histogram : NCI-H292 cells

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399