Calmodulin antibody [2D1]

Cat. No. GTX22860

GTX22860 FCM Image

FACS analysis of PC12 cells using GTX22860 Calmodulin antibody [2D1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

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GTX22860 FCM Image

FACS analysis of MCF-7 cells using GTX22860 Calmodulin antibody [2D1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

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GTX22860 IHC-Fr Image

IHC-Fr analysis of rat brain tissue using GTX22860 Calmodulin antibody [2D1].

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GTX22860 WB Image

WB analysis of purified calmodulin protein using GTX22860 Calmodulin antibody [2D1].

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GTX22860 FCM Image

FACS analysis of C6 cells using GTX22860 Calmodulin antibody [2D1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

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GTX22860 IHC-P Image

IHC-P analysis of rat testis tissue using GTX22860 Calmodulin antibody [2D1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

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GTX22860 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22860 Calmodulin antibody [2D1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

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GTX22860 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22860 Calmodulin antibody [2D1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

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GTX22860 IHC-P Image

IHC-P analysis of rat cerebellum tissue using GTX22860 Calmodulin antibody [2D1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

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GTX22860 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22860 Calmodulin antibody [2D1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

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This Calmodulin antibody [2D1] (GTX22860) is a mouse monoclonal antibody validated for detection of Calmodulin by WB, ICC/IF, IHC-P, IHC-Fr, FCM, ELISA, IHC, and Neutralizing /Inhibition. Reactive to human, mouse, rat, and other species.

Host

Mouse
Clonality

Monoclonal
Clone Name

2D1
Isotype

IgG1
Applications

WB ICC/IF IHC-P IHC-Fr FCM ELISA IHC Neutralizing /Inhibition
Reactivity

Human, Mouse, Rat, Bovine, Chicken, Fungi, Shrew, Bacteria