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Calmodulin antibody [2D1]

Anti-Calmodulin antibody [2D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22860
Anti-Calmodulin antibody [2D1] used in IHC (Paraffin sections) (IHC-P). GTX22860
Anti-Calmodulin antibody [2D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22860
Anti-Calmodulin antibody [2D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22860
Anti-Calmodulin antibody [2D1] used in IHC (Paraffin sections) (IHC-P). GTX22860
Anti-Calmodulin antibody [2D1] used in Flow cytometry (FACS). GTX22860
Anti-Calmodulin antibody [2D1] used in Western Blot (WB). GTX22860
Anti-Calmodulin antibody [2D1] used in IHC (Frozen sections) (IHC-Fr). GTX22860
Anti-Calmodulin antibody [2D1] used in Flow cytometry (FACS). GTX22860
Anti-Calmodulin antibody [2D1] used in Flow cytometry (FACS). GTX22860

Cat. No. GTX22860

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Bovine, Chicken, Fungi, Shrew, Bacteria
Package
200 μl ($399)
See all Calmodulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:50
IHC-P Assay dependent
IHC-Fr 1:20
FACS 2μg per 10⁶ cells
ELISA Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Product Note

This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). Addition of EGTA to buffers completely inhibits antibody staining.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dictyostelium discoideum

Immunogen

Calmodulin purified from Dictyostelium discoideum.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_384880

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calmodulin-like , CALM2

Background

Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Database

Research Area

DATA IMAGES

Anti-Calmodulin antibody [2D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22860

GTX22860 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22860 Calmodulin antibody [2D1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Calmodulin antibody [2D1] used in IHC (Paraffin sections) (IHC-P). GTX22860

GTX22860 IHC-P Image

IHC-P analysis of rat cerebellum tissue using GTX22860 Calmodulin antibody [2D1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Calmodulin antibody [2D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22860

GTX22860 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22860 Calmodulin antibody [2D1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Calmodulin antibody [2D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22860

GTX22860 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22860 Calmodulin antibody [2D1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Calmodulin antibody [2D1] used in IHC (Paraffin sections) (IHC-P). GTX22860

GTX22860 IHC-P Image

IHC-P analysis of rat testis tissue using GTX22860 Calmodulin antibody [2D1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Calmodulin antibody [2D1] used in Flow cytometry (FACS). GTX22860

GTX22860 FACS Image

FACS analysis of C6 cells using GTX22860 Calmodulin antibody [2D1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-Calmodulin antibody [2D1] used in Western Blot (WB). GTX22860

GTX22860 WB Image

WB analysis of purified calmodulin protein using GTX22860 Calmodulin antibody [2D1].

Anti-Calmodulin antibody [2D1] used in IHC (Frozen sections) (IHC-Fr). GTX22860

GTX22860 IHC-Fr Image

IHC-Fr analysis of rat brain tissue using GTX22860 Calmodulin antibody [2D1].

Anti-Calmodulin antibody [2D1] used in Flow cytometry (FACS). GTX22860

GTX22860 FACS Image

FACS analysis of MCF-7 cells using GTX22860 Calmodulin antibody [2D1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

Anti-Calmodulin antibody [2D1] used in Flow cytometry (FACS). GTX22860

GTX22860 FACS Image

FACS analysis of PC12 cells using GTX22860 Calmodulin antibody [2D1] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature

REFERENCE

Application Reference

Liu W et al. J Histochem Cytochem 2022; 70 (8) : 583-596 Expression of Calbindin-D28K in the Developing and Adult Mouse Cochlea.
Sui J et al. Mol Cell Proteomics 2008; 7 (6) : 1007-18 Comparative proteomics analysis of vascular smooth muscle cells incubated with S- and R-enantiomers of * using iTRAQ-coupled two-dimensional LC-MS/MS.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399