APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:1000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone J4D8 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Calmodulin protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calmodulin 2 , CALM2 , CAMII , LQT15 , PHKD , PHKD2 , caM , Calmodulin 2
Cellular Localization
Cytoplasm,Cytoskeleton,Spindle,Spindle pole
Background
This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]
Database
Research Area
DATA IMAGES
|
GTX57543 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57543 Calmodulin antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
|
|
GTX57543 FACS Image
FACS analysis of HeLa cells using GTX57543 Calmodulin antibody. Cell Number: 1 x 10⁶ cells Red: Primary antibody Antibody amount: 2-5 μg
|
|
GTX57543 WB Image
WB analysis of various samples using GTX57543 Calmodulin antibody. Lane 1 : NIH-3T3 whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : A549 whole cell lysate Lane 5 : MCF-7 whole cell lysate Lane 6 : Ramos whole cell lysate Lane 7 : PC-3 whole cell lysate Loading : 40 μg Dilution : 1:1000
|
REFERENCE
There are currently no references for Calmodulin antibody [J4D8] (GTX57543). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Calmodulin antibody [J4D8] (GTX57543). Be the first to share your experience with this product.