Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calmodulin antibody (GTX133805) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (C7 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calmodulin antibody (GTX133805) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.