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Calnexin antibody [3H4A7]

Anti-Calnexin antibody [3H4A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83045
Anti-Calnexin antibody [3H4A7] used in Western Blot (WB). GTX83045

Cat. No. GTX83045

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3H4A7

Isotype

 IgG2b

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($429)
See all Calnexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [3H4A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83045

GTX83045 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83045 Calnexin antibody [3H4A7].
Green : Calnexin
Red : Actin filament

Anti-Calnexin antibody [3H4A7] used in Western Blot (WB). GTX83045

GTX83045 WB Image

WB analysis of A431 (1), HeLa (2), MCF-7 (3) and A549 (4) cell lysate using GTX83045 Calnexin antibody [3H4A7].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 429