Calnexin antibody [AF18]

Cat No. GTX22798

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	AF18
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC
Reactivity	Human, Mouse, Rat, Primate
Package	100 μl ($339)

See all Calnexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:100-1:1000
ICC/IF	1:50-1:500
IHC-P	Assay dependent
IHC-Fr	1:20
IP	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Product Note

Immunocytochemical staining of calnexin with GTX22798 in Huh-7 cells yields a pattern consistent with specific endoplasmic reticulum staining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Focus human hepatoma cell lysate.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_368880

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Database

Gene ID: 821 CANX
UniProt: P27824 CANX

Research Area

DATA IMAGES

GTX22798 WB Image

WB analysis of 25 ug of HepG2 lysate using GTX22798 Calnexin antibody [AF18].
Dilution : 1:500

GTX22798 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX22798 Calnexin antibody [AF18].
Green : Primary antibody
Blue : Nuclei

GTX22798 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX22798 Calnexin antibody [AF18].
Red : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.05% Triton X-100 in PBS for 60 seconds at room temperature
Dilution : 1:100 for 2 hours at room temperature

GTX22798 IHC-P Image

IHC-P analysis of human hepatocarcinoma tissue using GTX22798 Calnexin antibody [AF18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX22798 IHC-P Image

IHC-P analysis of human kidney tissue using GTX22798 Calnexin antibody [AF18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

GTX22798 ICC/IF Image

ICC/IF analysis of A549 cells using GTX22798 Calnexin antibody [AF18].
Green : Primary antibody
Blue : Nuclei

GTX22798 ICC/IF Image

ICC/IF analysis of transfected COS7 cells using GTX22798 Calnexin antibody [AF18].
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:500

REFERENCE

Application Reference

Bunger S et al. Cytotechnology 2009; 61 (3) : 153-9 Comparison of five commercial extraction kits for subsequent membrane protein profiling.

Application : WB

Reactivity : Human

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REVIEW

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Application Reference

Bunger S et al. Cytotechnology 2009; 61 (3):153-9 Comparison of five commercial extraction kits for subsequent membrane protein profiling.

Application : WB

Reactivity : Human

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 339

Qty

Calnexin antibody [AF18]

Cat No. GTX22798

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX22798 WB Image

GTX22798 ICC/IF Image

GTX22798 ICC/IF Image

GTX22798 IHC-P Image

GTX22798 IHC-P Image

GTX22798 ICC/IF Image

GTX22798 ICC/IF Image

REFERENCE

Application Reference

REVIEW

LC3B antibody

Cyclin D1 antibody [N1C3]

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