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Calnexin antibody [AF18]

Anti-Calnexin antibody [AF18] used in Western Blot (WB). GTX22798
Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798
Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798
Anti-Calnexin antibody [AF18] used in IHC (Paraffin sections) (IHC-P). GTX22798
Anti-Calnexin antibody [AF18] used in IHC (Paraffin sections) (IHC-P). GTX22798
Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798
Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798

Cat No. GTX22798

Host

Mouse

Clonality

Monoclonal

Clone Name

AF18

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, IHC

Reactivity

Human, Mouse, Rat, Primate
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ICC/IF 1:50-1:500
IHC-P Assay dependent
IHC-Fr 1:20
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Product Note

Immunocytochemical staining of calnexin with GTX22798 in Huh-7 cells yields a pattern consistent with specific endoplasmic reticulum staining.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Focus human hepatoma cell lysate.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_368880

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [AF18] used in Western Blot (WB). GTX22798

GTX22798 WB Image

WB analysis of 25 ug of HepG2 lysate using GTX22798 Calnexin antibody [AF18].
Dilution : 1:500

Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798

GTX22798 ICC/IF Image

ICC/IF analysis of HMVEC Cells using GTX22798 Calnexin antibody [AF18].
Green : Primary antibody
Blue : Nuclei

Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798

GTX22798 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX22798 Calnexin antibody [AF18].
Red : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.05% Triton X-100 in PBS for 60 seconds at room temperature
Dilution : 1:100 for 2 hours at room temperature

Anti-Calnexin antibody [AF18] used in IHC (Paraffin sections) (IHC-P). GTX22798

GTX22798 IHC-P Image

IHC-P analysis of human hepatocarcinoma tissue using GTX22798 Calnexin antibody [AF18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Calnexin antibody [AF18] used in IHC (Paraffin sections) (IHC-P). GTX22798

GTX22798 IHC-P Image

IHC-P analysis of human kidney tissue using GTX22798 Calnexin antibody [AF18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798

GTX22798 ICC/IF Image

ICC/IF analysis of A549 cells using GTX22798 Calnexin antibody [AF18].
Green : Primary antibody
Blue : Nuclei

Anti-Calnexin antibody [AF18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22798

GTX22798 ICC/IF Image

ICC/IF analysis of transfected COS7 cells using GTX22798 Calnexin antibody [AF18].
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:500

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339