Immunocytochemical staining of calnexin with GTX22798 in Huh-7 cells yields a pattern consistent with specific endoplasmic reticulum staining.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Focus human hepatoma cell lysate.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_368880
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
ICC/IF analysis of HMVEC Cells using GTX22798 Calnexin antibody [AF18]. Green : Primary antibody Blue : Nuclei
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GTX22798 ICC/IF Image
ICC/IF analysis of transfected COS7 cells using GTX22798 Calnexin antibody [AF18]. Red : Primary antibody Permeabilization : 0.1% Triton X-100 Dilution : 1:500
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GTX22798 ICC/IF Image
ICC/IF analysis of A549 cells using GTX22798 Calnexin antibody [AF18]. Green : Primary antibody Blue : Nuclei
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GTX22798 WB Image
WB analysis of 25 ug of HepG2 lysate using GTX22798 Calnexin antibody [AF18]. Dilution : 1:500
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GTX22798 ICC/IF Image
ICC/IF analysis of U-87 MG cells using GTX22798 Calnexin antibody [AF18]. Red : Primary antibody Blue : Nuclei Fixation : 4% paraformaldehyde Permeabilization : 0.05% Triton X-100 in PBS for 60 seconds at room temperature Dilution : 1:100 for 2 hours at room temperature
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GTX22798 IHC-P Image
IHC-P analysis of human hepatocarcinoma tissue using GTX22798 Calnexin antibody [AF18]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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GTX22798 IHC-P Image
IHC-P analysis of human kidney tissue using GTX22798 Calnexin antibody [AF18]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50