APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT18B9 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Calnexin protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calnexin , CANX , CNX , IP90 , P90 , Calnexin
Cellular Localization
Endoplasmic reticulum membrane,Endoplasmic reticulum,Melanosome
Background
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
Database
Research Area
DATA IMAGES
![Anti-Calnexin antibody [AT18B9] used in Flow cytometry (FACS). GTX57717](/templates/default/js/lightbox/images/loading.gif "Anti-Calnexin antibody [AT18B9] used in Flow cytometry (FACS). GTX57717")
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GTX57717 FACS Image
FACS analysis of HeLa (upper) and A549 (lower) cells using GTX57717 Calnexin antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg
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GTX57717 WB Image
WB analysis of various samples using GTX57717 Calnexin antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : 293T whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : Jurkat whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57717 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57717 Calnexin antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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REFERENCE
There are currently no references for Calnexin antibody [AT18B9] (GTX57717). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Calnexin antibody [AT18B9] (GTX57717). Be the first to share your experience with this product.
![Anti-Calnexin antibody [AT18B9] used in Flow cytometry (FACS). GTX57717](/upload/website/prouct_img/small/GTX57717/GTX57717_20200204_FC_w_23061123_381.webp "Anti-Calnexin antibody [AT18B9] used in Flow cytometry (FACS). GTX57717")
![Anti-Calnexin antibody [AT18B9] used in Western Blot (WB). GTX57717](/upload/website/prouct_img/small/GTX57717/GTX57717_20200204_WB_w_23061123_330.webp "Anti-Calnexin antibody [AT18B9] used in Western Blot (WB). GTX57717")
![Anti-Calnexin antibody [AT18B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57717](/upload/website/prouct_img/small/GTX57717/GTX57717_20200204_IF1_w_23061123_132.webp "Anti-Calnexin antibody [AT18B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57717")
![Anti-Calnexin antibody [AT18B9] used in Flow cytometry (FACS). GTX57717](/upload/website/prouct_img/normal/GTX57717/GTX57717_20200204_FC_w_23061123_381.webp "Anti-Calnexin antibody [AT18B9] used in Flow cytometry (FACS). GTX57717")
![Anti-Calnexin antibody [AT18B9] used in Western Blot (WB). GTX57717](/upload/website/prouct_img/normal/GTX57717/GTX57717_20200204_WB_w_23061123_330.webp "Anti-Calnexin antibody [AT18B9] used in Western Blot (WB). GTX57717")
![Anti-Calnexin antibody [AT18B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57717](/upload/website/prouct_img/normal/GTX57717/GTX57717_20200204_IF1_w_23061123_132.webp "Anti-Calnexin antibody [AT18B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57717")