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Calnexin antibody [C3], C-term

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Immunoprecipitation (IP). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

Cat No. GTX109669

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Sheep
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , *KN73

Product Note

IP/MS validation was supported by references (PMID:30377396)

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949824

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Immunoprecipitation (IP). GTX109669

GTX109669 IP Image

Calnexin antibody immunoprecipitates Calnexin protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 30 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Calnexin protein by 2 μg of Calnexin antibody (GTX109669) 7.5% SDS-PAGE The immunoprecipitated Calnexin protein was detected by Calnexin antibody (GTX109669) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Calnexin antibody [C3], C-term detects Calnexin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:10000

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Calnexin(GTX109669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Calnexin antibody [C3], C-term Cat No. GTX109669

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (Dengue Nucleocapsid protein -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with calnexin antibody (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Skelet Muscle in 2014.PMID: 25530838
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28615694
Date : Anonymous submitted on 30-Apr-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells, KN73 cells
Amount used : 15μg
Blocking : Superblock blocking buffer in TBS, 24°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A: HEK293 cells, Lane B: KN73 cells
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169