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Calnexin antibody [C3], C-term

KO/KD Comparison Orthogonal Overexpression IP/MS
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Immunoprecipitation (IP). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669
Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

Cat. No. GTX109669

Reference 28
6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Sheep
Package
100 μl ($399),
25 μl ($169)
See all Calnexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T

Product Note

IP/MS validation was supported by references (PMID:30377396)

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949824

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Immunoprecipitation (IP). GTX109669

GTX109669 IP Image

Calnexin antibody immunoprecipitates Calnexin protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 30 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Calnexin protein by 2 μg of Calnexin antibody (GTX109669) 7.5% SDS-PAGE The immunoprecipitated Calnexin protein was detected by Calnexin antibody (GTX109669) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Calnexin antibody [C3], C-term detects Calnexin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:10000

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Calnexin(GTX109669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Zhao X et al. Front Cell Neurosci 2022; 16 : 1021592 Huntingtin exon 1 deletion does not alter the subcellular distribution of huntingtin and gene transcription in mice.
Ge Gao et al. Am J Cancer Res 2022; 12 (5) : 1934-1959 Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.
Application : WB
Reactivity : Human
Vultaggio-Poma V et al. Theranostics 2022; 12 (2) : 859-874 Extracellular ATP is increased by release of ATP-loaded microparticles triggered by nutrient deprivation.
Application : WB
Reactivity : Human
Pegoraro A et al. Cell Death Dis 2021; 12 (12) : 1088 P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells.
Application : WB
Reactivity : Mouse
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Calnexin antibody [C3], C-term Cat. No. GTX109669

Rating
( Average 5 based on 6 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 25898173
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Clin Med in 2019.PMID: 31167519
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Date : Anonymous submitted on 05-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (Dengue Nucleocapsid protein -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with calnexin antibody (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Skelet Muscle in 2014. PMID: 25530838
Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28615694
Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669
Date : Anonymous submitted on 30-Apr-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells, KN73 cells
Amount used : 15μg
Blocking : Superblock blocking buffer in TBS, 24°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A: HEK293 cells, Lane B: KN73 cells
Show More +
Submit a Review
Application Reference
Zhao X et al. Front Cell Neurosci 2022; 16:1021592 Huntingtin exon 1 deletion does not alter the subcellular distribution of huntingtin and gene transcription in mice.
Ge Gao et al. Am J Cancer Res 2022; 12 (5):1934-1959 Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.
Application : WB
Reactivity : Human
Vultaggio-Poma V et al. Theranostics 2022; 12 (2):859-874 Extracellular ATP is increased by release of ATP-loaded microparticles triggered by nutrient deprivation.
Application : WB
Reactivity : Human
Pegoraro A et al. Cell Death Dis 2021; 12 (12):1088 P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells.
Application : WB
Reactivity : Mouse
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
Application : WB
Reactivity : Mouse
Jonathan P Davies et al. ACS Infect Dis 2020; 6 (12):3174-3189 Comparative Multiplexed Interactomics of SARS-CoV-2 and Homologous Coronavirus Nonstructural Proteins Identifies Unique and Shared Host-Cell Dependencies.
Application : WB, IP
Reactivity : Human
Madison T Wright et al. Mol Cell Proteomics 2020; 20:100008 Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.
Application : WB
Reactivity : Human
Hanif A et al. Oncotarget 2020; 11 (23):2246-2258 Exploring the role of survivin in neuroendocrine neoplasms.
Gaballa R et al. Cancers (Basel) 2020; 12 (8) Exosomes-Mediated Transfer of Itga2 Promotes Migration and Invasion of Prostate Cancer Cells by Inducing Epithelial-Mesenchymal Transition.
Application : WB
Reactivity : Human
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Jiang Y et al. J Cell Mol Med 2020; 24 (7):4356-4361 Novel mutations in ADAMTS13 CUB domains cause abnormal pre-mRNA splicing and defective secretion of ADAMTS13.
Application : ICC/IF
Kuo HF et al. J Clin Med 2019; 8 (6) Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion.
Application : WB
Reactivity : Human, Rat
Inoue J et al. J Virol 2019; (Epub) siRNA Screening for the Small GTPase Rab Proteins Identifies that Rab5B is a Major Regulator of Hepatitis B Virus Production.
Application : WB, ICC/IF
Reactivity : Human
Huang WT et al. Cancer Lett 2019; 442:287-298 Pigment epithelium-derived factor inhibits lung cancer migration and invasion by upregulating exosomal thrombospondin 1.
Application : WB
Reactivity : Human
Kondo Y et al. JCI Insight 2018; 3 (14) Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking.
Sikorski K et al. Nat Methods 2018; 15 (11):909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Fan J et al. J Cell Biol 2017; 216 (12):4153-4164 cTAGE5 deletion in pancreatic β cells impairs proinsulin trafficking and insulin biogenesis in mice.
Chang HY et al. Sci Rep 2017; 7 (1):3515 Selective serotonin reuptake inhibitor, fluoxetine, impairs E-cadherin-mediated cell adhesion and alters calcium homeostasis in pancreatic beta cells.
Application : ICC/IF
Reactivity : Mouse
Morioka S et al. Exp Cell Res 2017; (Epub) Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages.
Application : WB
Reactivity : Mouse
Sugahara D et al. Glycobiology 2016; (Epub) Mouse intestinal niche cells express a distinct α1, 2-fucosylated glycan recognized by a lectin from Burkholderia cenocepacia.
Lopes da Fonseca T et al. Hum Mol Genet 2016; (Epub) A familial ATP13A2 mutation enhances alpha-synuclein aggregation and promotes cell death.
Miyauchi Y et al. Mol Pharmacol 2015; 88 (4):800-12 Suppression of Cytochrome P450 3A4 Function by UDP-Glucuronosyltransferase 2B7 through a Protein-Protein Interaction: Cooperative Roles of the Cytosolic Carboxyl-Terminal Domain and the Luminal Anchoring Region.
Application : WB
Reactivity : Human
Palmer DN et al. Biochim Biophys Acta 2015; 1852 (10 Pt B):2279-86 Recent studies of ovine neuronal ceroid lipofuscinoses from BARN, the Batten Animal Research Network.
Application : ICC/IF
Reactivity : Sheep
Sharma S et al. Nat Commun 2015; 6:6881 APOBEC3A cytidine deaminase induces RNA editing in monocytes and macrophages.
Application : WB
Reactivity : Human
De Palma C et al. Skelet Muscle 2014; 4 (1):22 Deficient nitric oxide signalling impairs skeletal muscle growth and performance: involvement of mitochondrial dysregulation.
Application : WB
Reactivity : Mouse
Luo YH et al. Chem Res Toxicol 2013; 26 (5):662-73 Cadmium-based quantum dot induced autophagy formation for cell survival via oxidative stress.
Application : ICC/IF
Reactivity : Mouse
Chen YT et al. PLoS One 2012; 7 (9):e46171 R26R-GR: a Cre-activable dual fluorescent protein reporter mouse.
Application : ICC/IF
Reactivity : Mouse
Holzer RG et al. Cell 2011; 147 (1):173-84 Saturated fatty acids induce c-Src clustering within membrane subdomains, leading to JNK activation.
Application : WB
Reactivity : Mouse
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