GeneTex
United States (US)

Calnexin antibody [C3], C-term

GTX109669_40450_20160707_WB_shRNA_watermark_19011507_599.jpg
GTX109669_41094_IP_19011507_702.jpg
GTX109669_41094_20171115_ICC_IF_R_19011507_930.jpg
GTX109669_39959_IFA_19011507_174.jpg
GTX109669_41094_WB_M_19011507_947.jpg
GTX109669_41605_IHC_M_19011507_696.jpg
GTX109669_41094_20171218_WB_19011507_281.jpg
GTX109669_IHC_19011507_957.jpg
GTX109669_41605_IHC_M_2_19011507_715.jpg
GTX109669_41094_20171218_WB_R_19011507_383.jpg

Cat No. GTX109669

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Sheep
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , *KN73

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377396)

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calnexin,Cnx,Ip90,P90,Canx

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX109669_40450_20160707_WB_shRNA_watermark_19011507_599.jpg

GTX109669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669_41094_IP_19011507_702.jpg

GTX109669 IP Image

Calnexin antibody immunoprecipitates Calnexin protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 30 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Calnexin protein by 2 μg of Calnexin antibody (GTX109669) 7.5% SDS-PAGE The immunoprecipitated Calnexin protein was detected by Calnexin antibody (GTX109669) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109669_41094_20171115_ICC_IF_R_19011507_930.jpg

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109669_39959_IFA_19011507_174.jpg

GTX109669 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Calnexin(GTX109669) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX109669_41094_WB_M_19011507_947.jpg

GTX109669 WB Image

Calnexin antibody [C3], C-term detects Calnexin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:10000

GTX109669_41605_IHC_M_19011507_696.jpg

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669_41094_20171218_WB_19011507_281.jpg

GTX109669 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669_IHC_19011507_957.jpg

GTX109669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Calnexin(GTX109669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669_41605_IHC_M_2_19011507_715.jpg

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669_41094_20171218_WB_R_19011507_383.jpg

GTX109669 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Calnexin antibody [C3], C-term Cat No. GTX109669

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 30-Apr-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells, KN73 cells
Amount used : 15μg
Blocking : Superblock blocking buffer in TBS, 24°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A: HEK293 cells, Lane B: KN73 cells
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169