Calnexin antibody [C3], C-term

Name: Calnexin antibody [C3], C-term
Brand: GeneTex
SKU: GTX109669
Price: 399.00 USD
Rating: 5 (6 reviews)

Anti-Calnexin antibody [C3], C-term used in Western Blot (WB). GTX109669

Anti-Calnexin antibody [C3], C-term used in Immunoprecipitation (IP). GTX109669

Anti-Calnexin antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109669

Anti-Calnexin antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109669

Cat. No. GTX109669

Reference 27

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Sheep
Package	100 μl ($399), 25 μl ($169)

See all Calnexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:500-1:1000

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T

Product Note

IP/MS validation was supported by references (PMID:30377396)

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949824

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Gene ID: 821 CANX
UniProt: P27824 CANX

Research Area

DATA IMAGES

GTX109669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 IP Image

Calnexin antibody immunoprecipitates Calnexin protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 30 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Calnexin protein by 2 μg of Calnexin antibody (GTX109669) 7.5% SDS-PAGE The immunoprecipitated Calnexin protein was detected by Calnexin antibody (GTX109669) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX109669 WB Image

Calnexin antibody [C3], C-term detects Calnexin protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:10000

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Calnexin(GTX109669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin antibody [C3], C-term (GTX109669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [C3], C-term (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109669 ICC/IF Image

Calnexin antibody [C3], C-term detects Calnexin protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109669 IHC-P Image

Calnexin antibody [C3], C-term detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ge Gao et al. Am J Cancer Res 2022; 12 (5) : 1934-1959 Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.

Application : WB

Reactivity : Human

Vultaggio-Poma V et al. Theranostics 2022; 12 (2) : 859-874 Extracellular ATP is increased by release of ATP-loaded microparticles triggered by nutrient deprivation.

Application : WB

Reactivity : Human

Pegoraro A et al. Cell Death Dis 2021; 12 (12) : 1088 P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells.

Application : WB

Reactivity : Mouse

Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.

Application : WB

Reactivity : Mouse

Madison T Wright et al. Mol Cell Proteomics 2020; 20 : 100008 Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.

Application : WB

Reactivity : Human

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Submit a Reference

REVIEW

Calnexin antibody [C3], C-term Cat. No. GTX109669

Rating

( Average 5 based on 6 users reviews)

Application

Western Blot(WB)		( Average 5 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 10-Aug-2021

Score :

Application Tested : WB

Sample Species : Hu

Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015.PMID: 25898173

Date : Anonymous submitted on 10-Aug-2021

Score :

Application Tested : WB

Sample Species : Hu

Figure Note : The data was peer reviewed and published in the journal J Clin Med in 2019.PMID: 31167519

Date : Anonymous submitted on 05-May-2021

Score :

Application Tested : WB

Sample Species : Hu

Sample : A549 cells

Amount used : 10 μg

Blocking : 5% skim milk in TBST, 27°C, 1Hr

Primary Antibody : 1:5000, 4°C, 12Hr

Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr

Lane Description : Two groups (Dengue Nucleocapsid protein -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with calnexin antibody (GTX109669) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.