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Calnexin antibody [CANX/1541]

Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446
Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446
Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446
Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446
Anti-Calnexin antibody [CANX/1541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34446
Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446

Cat No. GTX34446

Host

Mouse

Clonality

Monoclonal

Clone Name

CANX/1541

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF Assay dependent
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA 2-4μg/ml (for coating)

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Specificity/Sensitivity

We do not recommend use of this product for Rat.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CANX , CNX , IP90 , P90 , calnexin , Calnexin

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum,Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446

GTX34446 WB Image

WB analysis of human kidney tissue lysate using GTX34446 Calnexin antibody [CANX/1541].

Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446

GTX34446 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX34446 Calnexin antibody [CANX/1541].

Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446

GTX34446 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX34446 Calnexin antibody [CANX/1541].

Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446

GTX34446 IHC-P Image

IHC-P analysis of human small intestinal carcinoma tissue using GTX34446 Calnexin antibody [CANX/1541].

Anti-Calnexin antibody [CANX/1541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34446

GTX34446 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34446 Calnexin antibody [CANX/1541].
Green : Primary antibody
Red : nucleus

Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446

GTX34446 WB Image

WB analysis of HeLa and MCF-7 cell lysates using GTX34446 Calnexin antibody [CANX/1541].

REFERENCE

There are currently no references for Calnexin antibody [CANX/1541] (GTX34446). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Calnexin antibody [CANX/1541] (GTX34446). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf