APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| Assay dependent |
| 1-2μg/ml for 30 minutes at RT |
| 2-4μg/ml (for coating) |
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Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
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Not tested in other applications.
Calculated MW
Product Note
We do not recommend use of this product for Rat samples.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CANX , CNX , IP90 , P90 , calnexin , Calnexin
Cellular Localization
Endoplasmic reticulum membrane,Endoplasmic reticulum,Melanosome
Background
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
Database
Research Area
DATA IMAGES
![Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446](/templates/default/js/lightbox/images/loading.gif "Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446")
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GTX34446 IHC-P Image
IHC-P analysis of human small intestinal carcinoma tissue using GTX34446 Calnexin antibody [CANX/1541].
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GTX34446 WB Image
WB analysis of HeLa and MCF-7 cell lysates using GTX34446 Calnexin antibody [CANX/1541].
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GTX34446 WB Image
WB analysis of human kidney tissue lysate using GTX34446 Calnexin antibody [CANX/1541].
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GTX34446 IHC-P Image
IHC-P analysis of human renal cell carcinoma tissue using GTX34446 Calnexin antibody [CANX/1541].
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GTX34446 IHC-P Image
IHC-P analysis of human renal cell carcinoma tissue using GTX34446 Calnexin antibody [CANX/1541].
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GTX34446 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX34446 Calnexin antibody [CANX/1541].
Green : Primary antibody
Red : nucleus
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REFERENCE
There are currently no references for Calnexin antibody [CANX/1541] (GTX34446). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Calnexin antibody [CANX/1541] (GTX34446). Be the first to share your experience with this product.
![Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446](/upload/website/prouct_img/small/GTX34446/GTX34446_20200115_IHC-P_1145_w_23060801_296.webp "Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446](/upload/website/prouct_img/small/GTX34446/GTX34446_20200115_WB_107_w_23060801_859.webp "Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446](/upload/website/prouct_img/small/GTX34446/GTX34446_20200115_WB_658_w_23060801_962.webp "Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446](/upload/website/prouct_img/small/GTX34446/GTX34446_20200115_IHC-P_1062_w_23060801_714.webp "Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446](/upload/website/prouct_img/small/GTX34446/GTX34446_20200115_IHC-P_1061_w_23060801_873.webp "Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34446](/upload/website/prouct_img/small/GTX34446/GTX34446_20200115_ICC-IF_195_w_23060801_883.webp "Anti-Calnexin antibody [CANX/1541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446](/upload/website/prouct_img/normal/GTX34446/GTX34446_20200115_IHC-P_1145_w_23060801_296.webp "Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446](/upload/website/prouct_img/normal/GTX34446/GTX34446_20200115_WB_107_w_23060801_859.webp "Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446](/upload/website/prouct_img/normal/GTX34446/GTX34446_20200115_WB_658_w_23060801_962.webp "Anti-Calnexin antibody [CANX/1541] used in Western Blot (WB). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446](/upload/website/prouct_img/normal/GTX34446/GTX34446_20200115_IHC-P_1062_w_23060801_714.webp "Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446](/upload/website/prouct_img/normal/GTX34446/GTX34446_20200115_IHC-P_1061_w_23060801_873.webp "Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446")
![Anti-Calnexin antibody [CANX/1541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34446](/upload/website/prouct_img/normal/GTX34446/GTX34446_20200115_ICC-IF_195_w_23060801_883.webp "Anti-Calnexin antibody [CANX/1541] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34446")