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Calnexin antibody [CANX/1541]

Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446
Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446
Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446

Cat No. GTX34446

Host

Mouse

Clonality

Monoclonal

Clone Name

CANX/1541

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Specificity/Sensitivity

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Calnexin , Cnx , Ip90 , P90 , Canx

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446

GTX34446 IHC-P Image

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).

Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446

GTX34446 IHC-P Image

Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).

Anti-Calnexin antibody [CANX/1541] used in IHC (Paraffin sections) (IHC-P). GTX34446

GTX34446 IHC-P Image

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).

REFERENCE

There are currently no references for Calnexin antibody [CANX/1541] (GTX34446). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Calnexin antibody [CANX/1541] (GTX34446). Be the first to share your experience with this product.
Package List Price ($)
$ 429