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Calnexin antibody [CANX/1543]

Anti-Calnexin antibody [CANX/1543] used in Western Blot (WB). GTX17859
Anti-Calnexin antibody [CANX/1543] used in IHC (Paraffin sections) (IHC-P). GTX17859
Anti-Calnexin antibody [CANX/1543] used in Western Blot (WB). GTX17859
Anti-Calnexin antibody [CANX/1543] used in Protein Array (Protein Array). GTX17859

Cat No. GTX17859

Host

Mouse

Clonality

Monoclonal

Clone Name

CANX/1543

Isotype

IgG1

Application

WB, IHC-P, ELISA, Protein Array

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
ELISA 2-4μg/ml (for coating)
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CANX , CNX , IP90 , P90 , calnexin , Calnexin

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum,Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [CANX/1543] used in Western Blot (WB). GTX17859

GTX17859 WB Image

WB analysis of human kidney tissue lysate using GTX17859 Calnexin antibody [CANX/1543].

Anti-Calnexin antibody [CANX/1543] used in IHC (Paraffin sections) (IHC-P). GTX17859

GTX17859 IHC-P Image

IHC-P analysis of human renal cell carcinoma tissue using GTX17859 Calnexin antibody [CANX/1543].

Anti-Calnexin antibody [CANX/1543] used in Western Blot (WB). GTX17859

GTX17859 WB Image

WB analysis of PANC1 and MCF-7 cell lysates using GTX17859 Calnexin antibody [CANX/1543].

Anti-Calnexin antibody [CANX/1543] used in Protein Array (Protein Array). GTX17859

GTX17859 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Calnexin Mouse Monoclonal Antibody (CANX/1543). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379