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Calnexin antibody [GT1563]

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

Cat No. GTX629976

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1563

Isotype

IgG2a

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

mouse liver , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2

Product Note

IP/MS validation was supported by references (PMID:30377428)

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888183

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976

GTX629976 ICC/IF Image

Calnexin antibody [GT1563] detects Calnexin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500.
Red: Calnexin, stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:500

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169