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Calnexin antibody [GT1563]

KO/KD Overexpression IP/MS
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976
Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976
Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976

Cat. No. GTX629976

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1563

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, Dot

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calnexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
Dot Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

mouse liver , PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2

Product Note

IP/MS validation was supported by references (PMID:30377428)

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888183

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:500

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
Calnexin antibody [GT1563] (GTX629976) dilution: 1:1000

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Calnexin antibody [GT1563] used in Western Blot (WB). GTX629976

GTX629976 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976

GTX629976 ICC/IF Image

Calnexin antibody [GT1563] detects Calnexin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500.
Red: Calnexin, stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Calnexin antibody [GT1563] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629976

GTX629976 ICC/IF Image

Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [GT1563] (GTX629976) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Azalea A Khan et al. Bioconjug Chem 2022; 33 (3) : 473-485 PET Imaging of Small Extracellular Vesicles via [89Zr]Zr(oxinate)4 Direct Radiolabeling.
Application : Dot
Reactivity : Human
Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4) : e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.
Application : WB
AA Khan et al. Anal Chem 2020; [89Zr]Zr(oxinate)4 Allows Direct Radiolabelling and PET Imaging of Small Extracellular Vesicles
Gao Z et al. Antiviral Res 2020; 182 : 104907 Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations