Calnexin antibody [HL1598]

Name: Calnexin antibody [HL1598]
Brand: GeneTex
SKU: GTX637077
Price: 399.00 USD

Anti-Calnexin antibody [HL1598] used in Western Blot (WB). GTX637077

Anti-Calnexin antibody [HL1598] used in IHC (Paraffin sections) (IHC-P). GTX637077

Cat. No. GTX637077

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1598
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat, Cat, Dog
Package	100 μl ($399), 25 μl ($169)

See all Calnexin products

Datasheet

View product citations for antibody GTX637077 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Calnexin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Database

Gene ID: 821 CANX
UniProt: P27824 CANX

Research Area

DATA IMAGES

GTX637077 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637077 IHC-P Image

Calnexin antibody [HL1598] detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637077 IHC-P Image

Calnexin antibody [HL1598] detects Calnexin protein at endoplasmic reticulum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637077 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637077 IHC-P Image

Calnexin antibody [HL1598] detects Calnexin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637077 IHC-P Image

Calnexin antibody [HL1598] detects Calnexin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637077 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637077 IHC-P Image

Calnexin antibody [HL1598] detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog pancreas.
Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637077 IHC-P Image

Calnexin antibody [HL1598] detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
Calnexin stained by Calnexin antibody [HL1598] (GTX637077) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637077 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [HL1598] (GTX637077) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Calnexin antibody [HL1598]

Cat. No. GTX637077

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX637077 WB Image

GTX637077 IHC-P Image

GTX637077 IHC-P Image

GTX637077 WB Image

GTX637077 IHC-P Image

GTX637077 IHC-P Image

GTX637077 WB Image

GTX637077 IHC-P Image

GTX637077 IHC-P Image

GTX637077 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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