GeneTex
United States (US)

Calnexin antibody, Internal (near N-term)

GTX88369_992_IHC-P_18121411_182.jpg
GTX88369_991_IHC-P_18121411_617.jpg
GTX88369_990_IHC-P_18121411_316.jpg
GTX88369_19_ELISA_18121411_192.jpg
GTX88369_129_ICC-IF_18121411_741.jpg
GTX88369_2386_WB_18121411_915.jpg

Cat No. GTX88369

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, sELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
ICC/IF 1-2μg/ml
IHC-P 3.75μg/ml
ELISA Assay dependent
sELISA Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of NIH3T3 cells.

IHC-P
Human Kidney shows textured staining in the cytoplasm of DCT cells.

sELISA
Capture : GTX112886 , Detection : GTX88369

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88369-PEP).

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calnexin,Cnx,Ip90,P90,Canx

Cellular Localization

Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88369_992_IHC-P_18121411_182.jpg

GTX88369 IHC-P Image

IHC-P analysis of human kidney using GTX88369 Calnexin antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88369_991_IHC-P_18121411_617.jpg

GTX88369 IHC-P Image

IHC-P analysis of human uterus using GTX88369 Calnexin antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88369_990_IHC-P_18121411_316.jpg

GTX88369 IHC-P Image

IHC-P analysis of human testis using GTX88369 Calnexin antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88369_19_ELISA_18121411_192.jpg

GTX88369 ELISA Image

ELISA analysis of Calnexin protein using GTX88369 Calnexin antibody, Internal (near N-term).
Capture : GTX112886 (2.5μg/ml)
Detection : GTX88369 (1.5μg/ml)

GTX88369_129_ICC-IF_18121411_741.jpg

GTX88369 ICC/IF Image

ICC/IF analysis of PFA fixed NIH-3T3 cells using GTX88369 Calnexin antibody, Internal (near N-term).
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5µg/ml

GTX88369_2386_WB_18121411_915.jpg

GTX88369 WB Image

WB analysis of mouse heart lysate using GTX88369 Calnexin antibody, Internal (near N-term).
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329