APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 1-2μg/ml |
| 3.75μg/ml |
| Assay dependent |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of NIH3T3 cells.
IHC-P
Human Kidney shows textured staining in the cytoplasm of DCT cells.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88369-PEP).
Predict Reactivity
Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calnexin , CNX , IP90 , P90
Cellular Localization
Endoplasmic reticulum membrane , Endoplasmic reticulum
Background
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]
Database
Research Area
DATA IMAGES
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GTX88369 ICC/IF Image
ICC/IF analysis of PFA fixed NIH-3T3 cells using GTX88369 Calnexin antibody, Internal (near N-term).
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5μg/ml
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GTX88369 WB Image
WB analysis of mouse heart lysate using GTX88369 Calnexin antibody, Internal (near N-term).
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88369 IHC-P Image
IHC-P analysis of human testis using GTX88369 Calnexin antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX88369 IHC-P Image
IHC-P analysis of human uterus using GTX88369 Calnexin antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX88369 ELISA Image
ELISA analysis of Calnexin protein using GTX88369 Calnexin antibody, Internal (near N-term).
Capture : GTX112886 (2.5μg/ml)
Detection : GTX88369 (1.5μg/ml)
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GTX88369 IHC-P Image
IHC-P analysis of human kidney using GTX88369 Calnexin antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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REFERENCE
There are currently no references for Calnexin antibody, Internal (near N-term) (GTX88369). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Calnexin antibody, Internal (near N-term) (GTX88369). Be the first to share your experience with this product.
