Calnexin antibody [N3C2], Internal

Name: Calnexin antibody [N3C2], Internal
Brand: GeneTex
SKU: GTX101676
Price: 399.00 USD

Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

Anti-Calnexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101676

Cat. No. GTX101676

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Calnexin products

View product citations for antibody GTX101676 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

NIH-3T3 , PC-12 , Rat2 , 293T

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949820

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Gene ID: 821 CANX
UniProt: P27824 CANX

Research Area

DATA IMAGES

GTX101676 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX101676 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101676 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 ICC/IF Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX101676 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.