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Calnexin antibody [N3C2], Internal

Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676
Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

Cat No. GTX101676

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

calnexin,CNX,IP90,P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676

GTX101676 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101676

GTX101676 ICC/IF Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676

GTX101676 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676

GTX101676 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101676 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody [N3C2], Internal used in Western Blot (WB). GTX101676

GTX101676 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101676

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169