GeneTex
United States (US)

Calnexin antibody [N3C2], Internal

GTX101676_39897_20160707_WB_shRNA_watermark_19011506_645.jpg
GTX101676_39897_20150123_IHC_M_19011506_940.jpg
GTX101676_39897_IHC_19011506_669.jpg
GTX101676_39897_20160602_IFA_19011506_605.jpg
GTX101676_39897_20151231_WB_R_19011506_576.jpg
GTX101676_WB_M_19011506_557.jpg
GTX101676_43453_20190111_WB_19021719_553.jpg

Cat No. GTX101676

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calnexin,Cnx,Ip90,P90,Canx

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101676_39897_20160707_WB_shRNA_watermark_19011506_645.jpg

GTX101676 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676_39897_20150123_IHC_M_19011506_940.jpg

GTX101676 IHC-P Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101676_39897_IHC_19011506_669.jpg

GTX101676 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using Calnexin(GTX101676) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101676_39897_20160602_IFA_19011506_605.jpg

GTX101676 ICC/IF Image

Calnexin antibody [N3C2], Internal detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calnexin protein stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:200.
Blue: Hoechst 33342 staining.

GTX101676_39897_20151231_WB_R_19011506_576.jpg

GTX101676 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676_WB_M_19011506_557.jpg

GTX101676 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101676 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101676_43453_20190111_WB_19021719_553.jpg

GTX101676 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169