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Calnexin antibody

Anti-Calnexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00742
Anti-Calnexin antibody used in Immunoprecipitation (IP). GTX00742
Anti-Calnexin antibody used in IHC (Paraffin sections) (IHC-P). GTX00742
Anti-Calnexin antibody used in Western Blot (WB). GTX00742
Anti-Calnexin antibody used in Western Blot (WB). GTX00742
Anti-Calnexin antibody used in Western Blot (WB). GTX00742

Cat. No. GTX00742

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Calnexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100-1:300
IHC-P 1:100-1:1000
IP 1:100
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

C-terminal peptide of mouse Calnexin protein, EDEILNRSPRNRKPRRE, conjugated with KLH

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , 1110069N15Rik , AI988026 , Cnx , D11Ertd153e

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00742

GTX00742 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00742 Calnexin antibody.
Dilution : 1:100 or 1:300
Fixation : 4 % PFA at RT for 30 min or at 4 degree C for, overnight
Permeabilization : 0.25 % Triton X-100 in PBS for 10 min

Anti-Calnexin antibody used in Immunoprecipitation (IP). GTX00742

GTX00742 IP Image

IP analysis of mouse testis using GTX00742 Calnexin antibody.
Lane 1 : Input mouse testis lysate
Lane 2 : Precipitated with preimmune serum
Lane 3 : Precipitated with anti-CANX antibody
Total protein amount : 1 mg
IP antibody : 2 μg
IB antibody : 1:1000
Total protein amount : 1 mg

Anti-Calnexin antibody used in IHC (Paraffin sections) (IHC-P). GTX00742

GTX00742 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00742 Calnexin antibody.
Dilution : 1:100
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

Anti-Calnexin antibody used in Western Blot (WB). GTX00742

GTX00742 WB Image

WB analysis of mouse testis extracts of different ages (1-6 weeks) using GTX00742 Calnexin antibody.
Dilution : 1:1000
Loading : 20 μg

Anti-Calnexin antibody used in Western Blot (WB). GTX00742

GTX00742 WB Image

WB analysis of mouse testis or sperm tissue lysate using GTX00742 Calnexin antibody.
Dilution : 1:1000
Loading : 10 μg

Anti-Calnexin antibody used in Western Blot (WB). GTX00742

GTX00742 WB Image

WB analysis of various samples using GTX00742 Calnexin antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Dilution : 1:1000
Loading : 20 μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399