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Calnexin antibody

KO/KD Overexpression
Anti-Calnexin antibody used in Western Blot (WB). GTX112886
Anti-Calnexin antibody used in Western Blot (WB). GTX112886
Anti-Calnexin antibody used in Western Blot (WB). GTX112886
Anti-Calnexin antibody used in Western Blot (WB). GTX112886
Anti-Calnexin antibody used in IHC (Paraffin sections) (IHC-P). GTX112886
Anti-Calnexin antibody used in IHC (Paraffin sections) (IHC-P). GTX112886
Anti-Calnexin antibody used in Western Blot (WB). GTX112886
Anti-Calnexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112886
Anti-Calnexin antibody used in Western Blot (WB). GTX112886

Cat. No. GTX112886

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Calnexin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Mouse liver , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949822

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Melanosome

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody used in Western Blot (WB). GTX112886

GTX112886 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:10000.

Anti-Calnexin antibody used in Western Blot (WB). GTX112886

GTX112886 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody used in Western Blot (WB). GTX112886

GTX112886 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calnexin antibody used in Western Blot (WB). GTX112886

GTX112886 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX112886 diluted at 1:1000

Anti-Calnexin antibody used in IHC (Paraffin sections) (IHC-P). GTX112886

GTX112886 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calnexin(GTX112886) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calnexin antibody used in IHC (Paraffin sections) (IHC-P). GTX112886

GTX112886 IHC-P Image

Calnexin antibody detects Calnexin protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Calnexin antibody (GTX112886) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calnexin antibody used in Western Blot (WB). GTX112886

GTX112886 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX112886 diluted at 1:10000

Anti-Calnexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112886

GTX112886 ICC/IF Image

Calnexin antibody detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Calnexin protein stained by Calnexin antibody (GTX112886) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-Calnexin antibody used in Western Blot (WB). GTX112886

GTX112886 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:1000.

REFERENCE

Application Reference

J Pendiuk Goncalves et al. Biochim Biophys Acta Mol Cell Res 2023; 1871 (2) : 119641 High-throughput analysis of glycan sorting into extracellular vesicles
Application : WB
Guo P et al. Adv Funct Mater 2021; 31 (44) A facile magnetic extrusion method for preparing endosome-derived vesicles for cancer drug delivery.
Mun SJ et al. Stem Cells Int 2021; 2021 : 6686625 Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma.
Application : WB
Reactivity : Mouse
Busatto S et al. Pharmaceuticals (Basel) 2021; 14 (4) A Simple and Quick Method for Loading Proteins in Extracellular Vesicles.
Application : WB
Reactivity : Human
Guo P. et al. Advanced Functional Materials 2021; (Epub) A Facile Magnetic Extrusion Method for Preparing Endosome?Derived Vesicles for Cancer Drug Delivery.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169