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Calnexin antibody

Anti-Calnexin antibody used in Immunohistochemistry (IHC). GTX13504
Anti-Calnexin antibody used in Western Blot (WB). GTX13504

Cat No. GTX13504

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, FACS, IP, IHC

Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Bovine, Hamster, Guinea pig, Pig, Monkey, Xenopus laevis, Quail
Package
100 μl ($359)

APPLICATION

Application Note

FACS: Use at a dilution of 1/100. ICC: Use at a dilution of 1/200. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/2000 (this dilution was sufficient for detection of Calnexin in 20μg of HeLa heat shocked cell lysate). Detects a band of approximately 90 kDa. (predicted molecular weight: 71 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

68 kDa. ( Note )

Positive Control

Heat Shocked HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: Whole Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: EEDEILNRSPRNRKPRRE conjugated to KLH, corresponding to amino acids 575-593 of Human Calnexin.

Purification

Whole antiserum

Conjugation

Unconjugated

RRID

AB_368878

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Endoplasmic reticulum

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody used in Immunohistochemistry (IHC). GTX13504

GTX13504 IHC Image

Immunohistochemistry analysis of human spleen tissue stained with Calnexin, pAb at 10μg/ml.

Anti-Calnexin antibody used in Western Blot (WB). GTX13504

GTX13504 WB Image

"Western blot analysis: Lane 1: HeLa (heat shocked) Lane 2: Vero (heat shocked) Lane 3: Rat-2 (heat shocked) Lane 4: L-929 (heat shocked)."

REFERENCE

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Package List Price ($)
$ 359