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Calnexin antibody

Anti-Calnexin antibody used in Western Blot (WB). GTX13504
Anti-Calnexin antibody used in Immunohistochemistry (IHC). GTX13504

Cat. No. GTX13504

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Hamster, Guinea pig, Pig, Monkey, Xenopus laevis, Quail
Package
100 μl ($429)
See all Calnexin products

APPLICATION

Application Note

FACS: Use at a dilution of 1/100. ICC: Use at a dilution of 1/200. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/2000 (this dilution was sufficient for detection of Calnexin in 20μg of HeLa heat shocked cell lysate). Detects a band of approximately 90 kDa. (predicted molecular weight: 71 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

68 kDa. ( Note )

Positive Control

Heat Shocked HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: EEDEILNRSPRNRKPRRE conjugated to KLH, corresponding to amino acids 575-593 of Human Calnexin.

Purification

Whole antiserum

Conjugation

Unconjugated

RRID

AB_368878

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calnexin , CNX , IP90 , P90

Cellular Localization

Endoplasmic reticulum membrane , Endoplasmic reticulum

Background

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Database

Research Area

DATA IMAGES

Anti-Calnexin antibody used in Western Blot (WB). GTX13504

GTX13504 WB Image

"Western blot analysis: Lane 1: HeLa (heat shocked) Lane 2: Vero (heat shocked) Lane 3: Rat-2 (heat shocked) Lane 4: L-929 (heat shocked)."

Anti-Calnexin antibody used in Immunohistochemistry (IHC). GTX13504

GTX13504 IHC Image

Immunohistochemistry analysis of human spleen tissue stained with Calnexin, pAb at 10μg/ml.

REFERENCE

Application Reference

Amabile A et al. Cell 2016; 167 (1) : 219-232.e14 Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.
Application : WB
Reactivity : Human
Clayton P et al. Nucleus 2010; 1 (4) : 354-66 Mutations causing Greenberg dysplasia but not Pelger anomaly uncouple enzymatic from structural functions of a nuclear membrane protein.
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Package List Price ($)
$ 429