For ELISA: Use at a concentration of 0.5-1 μg/ml. IP: Use at a concentration of 1-2 μg/ml. For WB: Use at a concentration of 0.5-1 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user.
Recognizes the 80 kD subunit of μ-calpain as well as two smaller proteins that are presumed to be autolysis products.
50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5 containing 3mM Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Full length human protein
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mu Calpain, also known as Calpain I, and m calpain, also known as Calpain II, are intracellular, calcium-dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. The calpains have papain-like activity, thus the -pain nomenclature. Both m calpain and mu calpain are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin. Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV.