APPLICATION
Application Note
For ELISA: Use at a concentration of 0.5-1 μg/ml. IP: Use at a concentration of 1-2 μg/ml. For WB: Use at a concentration of 0.5-1 μg/ml. Detects a band of approximately 80 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Product Note
Recognizes the 80 kD subunit of μ-calpain as well as two smaller proteins that are presumed to be autolysis products.
PROPERTIES
Form
Liquid
Buffer
50% Glycerol, 0.15M Sodium Chloride, 0.02M Sodium Phosphate pH7.5
Preservative
3mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human protein
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calpain 1 , CANP , CANP1 , CANPL1 , SPG76 , muCANP , muCL
Cellular Localization
Cytoplasm , Cell membrane
Background
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Database
Research Area
REFERENCE
There are currently no references for Calpain 1 antibody [15C10] (GTX16680). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Calpain 1 antibody [15C10] (GTX16680). Be the first to share your experience with this product.