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Calpain 1 antibody [2H2A7C2]

Anti-Calpain 1 antibody [2H2A7C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25441
Anti-Calpain 1 antibody [2H2A7C2] used in Western Blot (WB). GTX25441
Anti-Calpain 1 antibody [2H2A7C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25441
Anti-Calpain 1 antibody [2H2A7C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25441

Cat. No. GTX25441

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H2A7C2

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Bovine, Pig
Package
100 μl ($399)
See all Calpain 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:20-1:25
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Product Note

This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. This antibody recognizes an epitope between amino acids 245-265 (domain II) of human mu-calpain.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bovine

Immunogen

Purified bovine skeletal muscle 80 kDa mu-calpain subunit.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 1 , CANP 1 , CANP1

Cellular Localization

Cytoplasm,Cell membrane

Database

Research Area

DATA IMAGES

Anti-Calpain 1 antibody [2H2A7C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25441

GTX25441 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX25441 Calpain 1 antibody [2H2A7C2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Calpain 1 antibody [2H2A7C2] used in Western Blot (WB). GTX25441

GTX25441 WB Image

WB analysis of mouse lung extract using GTX25441 Calpain 1 antibody [2H2A7C2].

Anti-Calpain 1 antibody [2H2A7C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25441

GTX25441 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX25441 Calpain 1 antibody [2H2A7C2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Calpain 1 antibody [2H2A7C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25441

GTX25441 ICC/IF Image

ICC/IF analysis of U251 cells using GTX25441 Calpain 1 antibody [2H2A7C2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399