ICC/IF analysis of U251 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

WB analysis of membrane enriched extracts (30 μg lysate) of HeLa (Lane 1), A-431 (Lane 2), MOLT-4 (Lane 3), SK-OV-3 (Lane 4), LNCaP (Lane 5) and PC-3 (Lane 6) using GTX23589 Calpain 1 antibody [9A4H8D3].
Dilution : 2 μg/ml

ICC/IF analysis of MCF-7 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

IHC-P analysis of human kidney tissue using GTX23589 Calpain 1 antibody [9A4H8D3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

WB analysis of 15 ug of HeLa cell lysate (lane 2), 15 ug of A431 cell lysate (lane 3) using GTX23589 Calpain 1 antibody [9A4H8D3].
Dilution : 1:500

IHC-P analysis of human tonsil tissue using GTX23589 Calpain 1 antibody [9A4H8D3].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

ICC/IF analysis of HeLa cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC