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Calpain 1 antibody [N3C2], Internal

Orthogonal
Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340

Cat. No. GTX102340

Reference 8
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Squirrel
Package
100 μl ($399),
25 μl ($169)
See all Calpain 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618731

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 1 , CANP , CANP1 , CANPL1 , SPG76 , muCANP , muCL

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340

GTX102340 IHC-P Image

Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Calpain 1 antibody [N3C2], Internal (GTX102340) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102340

GTX102340 IP Image

Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal (GTX102340).
Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal (GTX102340).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340

GTX102340 WB Image

Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody (GTX102340) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340

GTX102340 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1(GTX102340) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340

GTX102340 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Calpain 1(GTX102340) antibody at 1:200 dilution.

Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340

GTX102340 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35163312

REFERENCE

Application Reference

E Fletcher et al. Transl Res 2023; S1931-5244 (23) : 00088-9 Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
Application : WB
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Yang HH et al. Int J Mol Sci 2022; 23 (3) Anti-Excitotoxic Effects of N-Butylidenephthalide Revealed by Chemically Insulted Purkinje Progenitor Cells Derived from SCA3 iPSCs.
Application : WB
Reactivity : Human
Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Lin JH et al. Phytother Res 2020;
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Calpain 1 antibody [N3C2], Internal Cat. No. GTX102340

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Submit a Review
Application Reference
E Fletcher et al. Transl Res 2023; S1931-5244 (23):00088-9 Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease
Application : WB
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Yang HH et al. Int J Mol Sci 2022; 23 (3) Anti-Excitotoxic Effects of N-Butylidenephthalide Revealed by Chemically Insulted Purkinje Progenitor Cells Derived from SCA3 iPSCs.
Application : WB
Reactivity : Human
Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Lin JH et al. Phytother Res 2020;
Application : WB
Reactivity : Human
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Yang HH et al. Proteomics Clin Appl 2016; (Epub) the putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of * cystitis - revealed by a prospective pilot study using proteomic approaches.
Application : WB
Reactivity : Human
Zhang Y et al. Cell Stress Chaperones 2016; (Epub) Regulation of gene expression by NFAT transcription factors in hibernating ground squirrels is dependent on the cellular environment.
Application : WB
Reactivity : Squirrel
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169