GeneTex
United States (US)

Calpain 1 antibody [N3C2], Internal

GTX102340_40302_IHC_19011506_731.jpg
GTX102340_40302_IFA_19011506_834.jpg
GTX102340_40302_20150910_IP_19011506_438.jpg
GTX102340_40302_20150529_WB_19011506_367.jpg
GTX102340_40302_20150313_IHC_M_19011506_913.jpg

Cat No. GTX102340

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Squirrel
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calpain 1,Canp,Canp1,Canpl1,Spg76,Mucanp,Mucl,Capn1

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Database

DATA IMAGES
GTX102340_40302_IHC_19011506_731.jpg

GTX102340 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1(GTX102340) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102340_40302_IFA_19011506_834.jpg

GTX102340 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Calpain 1(GTX102340) antibody at 1:200 dilution.

GTX102340_40302_20150910_IP_19011506_438.jpg

GTX102340 IP Image

Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal (GTX102340).
Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal (GTX102340).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX102340_40302_20150529_WB_19011506_367.jpg

GTX102340 WB Image

Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody (GTX102340) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102340_40302_20150313_IHC_M_19011506_913.jpg

GTX102340 IHC-P Image

Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Calpain 1 antibody [N3C2], Internal (GTX102340) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169