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Calpain 1 antibody [N3C2], Internal

Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340
Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340

Cat. No. GTX102340

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Squirrel
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618731

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 1 , CANP , CANP1 , CANPL1 , SPG76 , muCANP , muCL

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340

GTX102340 IHC-P Image

Calpain 1 antibody [N3C2], Internal detects Calpain 1 protein at cytoplasm on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Calpain 1 antibody [N3C2], Internal (GTX102340) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody [N3C2], Internal used in Immunoprecipitation (IP). GTX102340

GTX102340 IP Image

Immunoprecipitation of Calpain 1 protein from A431 whole cell extracts using 5 μg of Calpain 1 antibody [N3C2], Internal (GTX102340).
Western blot analysis was performed using Calpain 1 antibody [N3C2], Internal (GTX102340).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Calpain 1 antibody [N3C2], Internal used in Western Blot (WB). GTX102340

GTX102340 WB Image

Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody (GTX102340) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX102340

GTX102340 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Calpain 1(GTX102340) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102340

GTX102340 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Calpain 1(GTX102340) antibody at 1:200 dilution.

REFERENCE

REVIEW

Calpain 1 antibody [N3C2], Internal Cat. No. GTX102340

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 24-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2022 in Int J Mol Sci. PMID: 35163312
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Application Reference
Lin JH et al. Phytother Res 2020;
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169