GeneTex
United States (US)

Calpain 1 antibody

GTX102350_40394_IHC_19011506_858.jpg
GTX102350_40394_IFA_19011506_878.jpg
GTX102350_40394_WB_19011506_649.jpg

Cat No. GTX102350

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A431 , Jurkat

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calpain 1,Canp,Canp1,Canpl1,Spg76,Mucanp,Mucl,Capn1

Cellular Localization

Cytoplasm , Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Database

DATA IMAGES
GTX102350_40394_IHC_19011506_858.jpg

GTX102350 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1(GTX102350) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102350_40394_IFA_19011506_878.jpg

GTX102350 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1(GTX102350) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX102350_40394_WB_19011506_649.jpg

GTX102350 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX102350 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169