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Calpain 1 antibody

Anti-Calpain 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102350
Anti-Calpain 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102350
Anti-Calpain 1 antibody used in Western Blot (WB). GTX102350

Cat No. GTX102350

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A431 , Jurkat

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

calpain 1 , CANP , CANP1 , CANPL1 , SPG76 , muCANP , muCL

Cellular Localization

Cytoplasm , Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Database

DATA IMAGES
Anti-Calpain 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102350

GTX102350 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1(GTX102350) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102350

GTX102350 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1(GTX102350) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Calpain 1 antibody used in Western Blot (WB). GTX102350

GTX102350 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX102350 diluted at 1:1000

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169