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Calpain 1 antibody

Anti-Calpain 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102350
Anti-Calpain 1 antibody used in Western Blot (WB). GTX102350
Anti-Calpain 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102350
Anti-Calpain 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102350
Anti-Calpain 1 antibody used in Western Blot (WB). GTX102350

Cat. No. GTX102350

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Calpain 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729181

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 1 , CANP , CANP1 , CANPL1 , SPG76 , muCANP , muCL

Cellular Localization

Cytoplasm , Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102350

GTX102350 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Calpain 1(GTX102350) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Calpain 1 antibody used in Western Blot (WB). GTX102350

GTX102350 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX102350 diluted at 1:1000

Anti-Calpain 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102350

GTX102350 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using Calpain 1(GTX102350) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102350

GTX102350 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calpain 1 antibody (GTX102350) at 1:300 dilution.

Anti-Calpain 1 antibody used in Western Blot (WB). GTX102350

GTX102350 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
7.5% SDS PAGE
GTX102350 diluted at 1:1000

REFERENCE

Application Reference

Mikosik A et al. Immun Ageing 2013; 10 (1) : 27 Expression of calpain-calpastatin system (CCS) member proteins in human lymphocytes of young and elderly individuals; pilot baseline data for the CALPACENT project.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169