Calpain 2 antibody [N2C1-2], Internal

Name: Calpain 2 antibody [N2C1-2], Internal
Brand: GeneTex
SKU: GTX111809
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Calpain 2 antibody [N2C1-2], Internal used in Western Blot (WB). GTX111809

Anti-Calpain 2 antibody [N2C1-2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111809

Cat. No. GTX111809

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Calpain 2 products

View product citations for antibody GTX111809 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10727594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 2 , CANP2 , CANPL2 , CANPml , mCANP

Cellular Localization

Cytoplasm , Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 824 CAPN2
UniProt: P17655 CAPN2

Research Area

DATA IMAGES

GTX111809 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111809 diluted at 1:1000

GTX111809 IHC-P Image

Calpain 2 antibody [N2C1-2], Internal detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Calpain 2 antibody [N2C1-2], Internal (GTX111809) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111809 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Calpain 2(GTX111809) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111809 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 2 antibody [N2C1-2], Internal (GTX111809) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111809 WB Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35163312

REFERENCE

Application Reference

Yang HH et al. Int J Mol Sci 2022; 23 (3) Anti-Excitotoxic Effects of N-Butylidenephthalide Revealed by Chemically Insulted Purkinje Progenitor Cells Derived from SCA3 iPSCs.

Application : WB

Reactivity : Human

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