GeneTex
United States (US)

Calpain 2 antibody [N2C1], Internal

GTX102499_WB_M_19011506_730.jpg
GTX102499_40163_IHC_M_19011506_658.jpg
GTX102499_IFA_19011506_179.jpg
GTX102499_WB_19011506_780.jpg

Cat No. GTX102499

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Jurkat , NIH-3T3

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CANP2 antibody, CANPL2 antibody, CANPml antibody, FLJ39928 antibody, mCANP antibody, CAPN2 antibody, calpain, large polypeptide L2 antibody, calpain 2, large [catalytic] subunit antibody, calpain-2 large subunit antibody, calpain 2, large subunit antibody, millimolar-calpain antibody, M-calpain antibody, CANP 2 antibody, calpain-2 catalytic subunit antibody, calcium-activated neutral proteinase 2 antibody, calpain large polypeptide L2 antibody, calpain M-type antibody, calpain 2, (m/II) large subunit antibody

Cellular Localization

Cytoplasm , Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX102499_WB_M_19011506_730.jpg

GTX102499 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX102499 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102499_40163_IHC_M_19011506_658.jpg

GTX102499 IHC-P Image

Calpain 2 antibody [N2C1], Internal detects Calpain 2 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Calpain 2 antibody [N2C1], Internal (GTX102499) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102499_IFA_19011506_179.jpg

GTX102499 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calpain 2(GTX102499) antibody at 1:200 dilution.

GTX102499_WB_19011506_780.jpg

GTX102499 WB Image

Sample (30 µg of whole cell lysate)
A: JurKat
7.5% SDS PAGE
Calpain 2 antibody
GTX102499 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
REVIEW

Calpain 2 antibody [N2C1], Internal Cat No. GTX102499

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 18-Dec-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse heart tissue
Amount used : 6 μg
Blocking : 3% BSA in TBST, 23°C, 1Hr
Primary Antibody : 1:5000, 23°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf