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Calpain 2 antibody [N2C1], Internal

Anti-Calpain 2 antibody [N2C1], Internal used in Western Blot (WB). GTX102499
Anti-Calpain 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102499
Anti-Calpain 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102499
Anti-Calpain 2 antibody [N2C1], Internal used in Western Blot (WB). GTX102499

Cat No. GTX102499

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Jurkat , NIH-3T3

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

calpain 2 , CANP2 , CANPL2 , CANPml , mCANP

Cellular Localization

Cytoplasm , Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Calpain 2 antibody [N2C1], Internal used in Western Blot (WB). GTX102499

GTX102499 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3 7.5% SDS PAGE
GTX102499 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Calpain 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102499

GTX102499 IHC-P Image

Calpain 2 antibody [N2C1], Internal detects Calpain 2 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Calpain 2 antibody [N2C1], Internal (GTX102499) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Calpain 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102499

GTX102499 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calpain 2(GTX102499) antibody at 1:200 dilution.

Anti-Calpain 2 antibody [N2C1], Internal used in Western Blot (WB). GTX102499

GTX102499 WB Image

Sample (30 µg of whole cell lysate)
A: JurKat
7.5% SDS PAGE
Calpain 2 antibody
GTX102499 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Calpain 2 antibody [N2C1], Internal Cat No. GTX102499

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 13-Jul-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC
Amount used : 20 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Date : Anonymous submitted on 18-Dec-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse heart tissue
Amount used : 6 μg
Blocking : 3% BSA in TBST, 23°C, 1Hr
Primary Antibody : 1:5000, 23°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169