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Calpain 2 antibody

KO/KD
Anti-Calpain 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55545
Anti-Calpain 2 antibody used in Western Blot (WB). GTX55545
Anti-Calpain 2 antibody used in Western Blot (WB). GTX55545

Cat. No. GTX55545

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Calpain 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human CAPN2 (NP_001739.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 2 , CANP2 , CANPL2 , CANPml , mCANP

Cellular Localization

Cytoplasm,Cell membrane

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Calpain 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55545

GTX55545 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX55545 Calpain 2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Calpain 2 antibody used in Western Blot (WB). GTX55545

GTX55545 WB Image

WB analysis of various sample lysates using GTX55545 Calpain 2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Calpain 2 antibody used in Western Blot (WB). GTX55545

GTX55545 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX55545 Calpain 2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399