*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
This antibody reacts with full-size calpain 3 (94 kD) plus an additional fragment at 30 kD in human skeletal muscle. A band of 94 kD is seen with rabbit and dog muscle while extracts of hamster muscle show reactivity with the 94 kD band and a larger species of approximately 110 kD. This 110 kD band is the principal immunoreactive species seen in rat muscle extracts. This antibody produces no bands with mouse, pig or chicken muscle.
We do not recommend use of this product for Mouse,Chicken,Pig samples.
Tissue culture supernatant
15mM Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
Synthetic peptide containing amino acids 1–19 of the human calpain 3 sequence.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
calpain 3 , CANP3 , CANPL3 , LGMD2 , LGMD2A , LGMDD4 , LGMDR1 , nCL-1 , p94
Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008]
GTX01971 WB Image
WB analysis of human skeletal muscle tissue lysate using GTX01970 Calpain 3 antibody [Calp3c/12A2] and GTX01970 Calpain 3 antibody [Calp3d/2C4].
Lane A : GTX01971
Lane B : GTX01970