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Calpain 3 antibody [Calp3d/2C4]

Anti-Calpain 3 antibody [Calp3d/2C4] used in Western Blot (WB). GTX01971

Cat. No. GTX01971

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Calp3d/2C4

Isotype

 IgG2b

Application

 WB

Reactivity

 Human, Rat, Rabbit, Dog, Hamster
Package
500 μl ($399)
See all Calpain 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:25–1:50
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Product Note

This antibody reacts with full-size calpain 3 (94 kD) plus an additional fragment at 30 kD in human skeletal muscle. A band of 94 kD is seen with rabbit and dog muscle while extracts of hamster muscle show reactivity with the 94 kD band and a larger species of approximately 110 kD. This 110 kD band is the principal immunoreactive species seen in rat muscle extracts. This antibody produces no bands with mouse, pig or chicken muscle.
We do not recommend use of this product for Mouse,Chicken,Pig samples.

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide containing amino acids 1–19 of the human calpain 3 sequence.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 3 , CANP3 , CANPL3 , LGMD2 , LGMD2A , LGMDD4 , LGMDR1 , nCL-1 , p94

Cellular Localization

Cytoplasm

Background

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Calpain 3 antibody [Calp3d/2C4] used in Western Blot (WB). GTX01971

GTX01971 WB Image

WB analysis of human skeletal muscle tissue lysate using GTX01970 Calpain 3 antibody [Calp3c/12A2] and GTX01970 Calpain 3 antibody [Calp3d/2C4].
Lane A : GTX01971
Lane B : GTX01970

REFERENCE

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REVIEW

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Package List Price ($)
$ 399