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Calpain 3 antibody

Anti-Calpain 3 antibody used in Western Blot (WB). GTX66789

Cat No. GTX66789

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to residues in Human CAPN3

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

calpain 3 , CANP3 , CANPL3 , LGMD2 , LGMD2A , LGMDD4 , LGMDR1 , nCL-1 , p94

Cellular Localization

Cytoplasm

Background

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Calpain 3 antibody used in Western Blot (WB). GTX66789

GTX66789 WB Image

WB analysis of various samples using GTX66789 Calpain 3 antibody.
Lane1 : MCF-7 whole cell lysate
Lane2 : PC12 whole cell lysate
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf